Technology for enriching soybean sprout isoflavone

A technology for isoflavones and soybean sprouts, which is applied to the field of isoflavone enrichment in soybean sprouts, can solve the problems of long enrichment period, complicated operation and high production cost, and achieves the effects of short enrichment period, simple operation and low production cost.

Inactive Publication Date: 2016-12-07
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to provide a production process of soybean sprouts rich in isoflavones, which is different from the traditional production process of enriching isoflavones, which can solve the p

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Use vigorous soybean grains as raw materials, sterilize them with sodium hypochlorite aqueous solution according to known methods, then place them in 3 to 5 times the volume (v / w) of pure water, soak them at 30°C for 5 hours, and put the grains into a germination machine Germinated at 30°C for 4 days, UV-B continuous radiation (wavelength 280nm, radiation distance 50cm, radiation intensity 20μW·cm -2 ) Use 15μMABA solution as the spray solution. The isoflavone content in soybean sprouts is about 4120μg / g DW.

Embodiment 2

[0022] Soybean grain disinfection, soaking method is the same as embodiment 1. Put the seeds into a germination machine, germinate at 30°C for 4 days, and irradiate continuously with UV-B (wavelength: 290nm, radiation distance: 45cm, radiation intensity: 25μW·cm -2 ) The lower spray liquid uses 3mM SNP solution. The isoflavone content in soybean sprouts is about 4580μg / g DW.

Embodiment 3

[0024] Soybean grain disinfection, soaking method is the same as embodiment 1. Put the seeds into a germination machine, germinate at 30°C for 4 days, and irradiate continuously with UV-B (wavelength: 300nm, radiation distance: 40cm, radiation intensity: 30μW·cm -2 ) The lower spray liquid uses 5 μ MABA and 2 mM SNP solution. The isoflavone content in soybean sprouts is about 5150μg / g DW.

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Abstract

The invention relates to a technology for enriching soybean sprout isoflavone. The technology adopting physiologically active soybean grains as a raw material comprises the following steps: disinfecting the raw materials in an aqueous sodium hypochlorite solution through a known method, placing the disinfected raw material in purified water with according to a ratio of 3-5:1 (v/w), immersing the raw material at 30 DEG C for 5 h, placing the immersed raw material in a germinator, and germinating the raw material at 30 DEG C; and continuously radiating medium wave ultraviolet lights (UV-B) (with the wavelength of 280-320 nm, the radiation distance of 30-50 cm and the radiation distance of 20-50 [mu]W.cm<-2>) on the germinated soybeans for 2-4 d in the germination period, and carrying out spray germination with a 0-15 [mu]M abscisic acid (ABA) and 0-3 mM sodium nitroprusside (SNP) mixed aqueous solution for 1-3 min every 1-4 h, wherein the flow amount of the above spraying liquid is 50-200 mL/min. The technology is simple to operate, and allows the content of isoflavone in the soybean sprouts to reach 4000-5500 [mu]g/g DW.

Description

1. Technical field [0001] The invention relates to a technique for enriching isoflavones in soybean sprouts, belonging to the technical field of food production. 2. Background technology [0002] Soybean grains are rich in nutrients such as protein, fat, and carbohydrates, as well as functional substances such as isoflavones and γ-aminobutyric acid. However, the content of isoflavones in soybean grains is only 0.2-1.6 mg·g -1 (DW). After soybean seeds germinate, the key enzymes of isoflavone synthesis are activated, and thus isoflavones accumulate. Isoflavones have various physiological effects on the human body, such as estrogenic properties, anti-cancer, reducing the incidence of cardiovascular diseases, and regulating immune responses. The use of biotransformation technology can further increase the content of isoflavones to expand its application range. [0003] The synthesis of secondary metabolites in plants is the result of resistance to adversity stress. It has b...

Claims

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Application Information

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IPC IPC(8): A23L11/20A23L5/10A23L33/00A23L11/70
CPCA23V2002/00A23V2250/2116
Inventor 顾振新焦彩凤杨润强
Owner NANJING AGRICULTURAL UNIVERSITY
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