Application of Bacillus pumilus to prevention and treatment of diseases of ganoderma due to Trichoderma atroviride
A technology of Bacillus pumilus and Trichoderma dark viridans, applied in application, fungicides, chemicals for biological control, etc., can solve the problems of poor control effect of biocontrol agents
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Embodiment 1
[0012] Use Bacillus pumilus to make wettable powder or granular inoculum according to the conventional biological control preparation process to prevent and control the disease of Ganoderma lucidum dark green. The specific test process is as follows:
[0013] 1. Source of the strain
[0014] 1.1 Source of pathogenic bacteria
[0015] In the Ganoderma lucidum cultivation base of Kangzhiyuan Ganoderma lucidum professional cooperative in Huangsongdian Town, Jiaohe, Jilin City, the pathogenic strains were obtained after the hyphae in the base were isolated and the back-grafting test followed Koch's law. PDA medium was used to cultivate pathogenic bacteria (recipe: 200g fresh potato, 1.0g glucose, 20g agar powder, H 2 O1000mL, pH natural), the medium was sterilized at 121°C for 25min before use.
[0016] 1.2 Source of bacterial strains
[0017] The soil was isolated from the surrounding soil of Ganoderma lucidum cultivation base in Kangzhi Garden Ganoderma lucidum professional c...
Embodiment 2
[0037] Various biological control bacteria agents such as wettable powder or granule can be made by using Bacillus pumilus.
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