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A molecular marker of structural variation sv141 for identifying Xiang pig breeds and its application

A technology of SV141 and structural variation, which is applied to the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of increasing the risk of follicular cysts in sows, and achieve the effect of simple operation, accurate and reliable method

Active Publication Date: 2019-12-13
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Structural variations (SVs) widely exist in mammalian genomes. Wanhong Li et al. (Liwanhong.et al, 2015) used PCR technology to detect inhibin α subunit gene fragments in Large White pig genomic DNA and found that inhibin α subunit gene There is a structural variation of 283bp, when 283bp exists, it will increase the risk of follicular cysts in sows

Method used

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  • A molecular marker of structural variation sv141 for identifying Xiang pig breeds and its application
  • A molecular marker of structural variation sv141 for identifying Xiang pig breeds and its application
  • A molecular marker of structural variation sv141 for identifying Xiang pig breeds and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 The detection technology of porcine structural variation SV141, the steps are as follows:

[0030] 1. Genomic DNA extraction

[0031] The blood / cell / tissue genomic DNA extraction kit of Tiangen Biochemical Technology (Beijing) Co., Ltd. was used to extract genomic DNA from blood or ear tissue. The specific method is as follows:

[0032] (1) Processing materials

[0033] When the extraction material is blood, 200 μL of fresh, frozen or blood with various anticoagulants can be taken directly, and buffer GA can be added to make up the volume if it is less than 200 μL.

[0034] When the extraction material is ear tissue, take 50 mg sample, cut it into pieces, put it in a 1.5 mL EP tube, add 200 μl buffer GA, and oscillate to fully suspend the tissue particles.

[0035] (2) Add 20 μL of proteinase K and vortex to mix. If it is a blood sample, continue to step (3); if it is an ear tissue, put it in a 56°C water bath until all the tissue fragments are dissolved, an...

Embodiment 2

[0051] Example 2 Application of Molecular Marker SV141

[0052] According to the method of Example 1, the test materials are as shown in Table 1, with chr14: 81642734-81643166 as the candidate structural variation, using PCR technology to detect the distribution of the genotype of the structural variation in 7 pig breed populations, using SPSSv20. The χ2 test in 0 software was used to analyze the significance of the difference of the gene frequency of the structural variation between Xiang pig and other pig breeds. The results show that the structural variation is dominated by the "deletion" type (D) in the Xiang pig population, and the "normal" type (A) in other pig breeds, and the gene frequency difference between the Xiang pig and other pig breeds is very large. Significantly (P figure 2 shown.

[0053] Table 1 Statistical table of test materials and their sources

[0054] pig breed Number of samples Blood / tissue collection source Fragrant pig 695 Co...

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Abstract

The invention discloses a molecular marker for identifying a structural variation SV141 of Xiang pig breeds. The molecular marker is characterized in that the structural variation SV141 is on chr14:81642733-81643165 of a pig reference genome (Ssc 10.2) and in an intron 1 of a mitochondrial calcium uniporter (MCU) gene, the structural variation is named as two genes of D or A, the three genotypes are respectively named as DD, DA and AA, the frequency of the gene D in a Xiang pig is remarkably higher than that in the other six pig breeds, and therefore, the gene can be serve as the molecular marker for identifying the Xiang pig breeds.

Description

technical field [0001] The invention relates to the field of livestock molecular biology detection, in particular to a molecular marker and application technology for identifying fragrant pig breeds. Background technique [0002] Congjiang Fragrant Pig is a precious local pig breed in my country. The production area is located in Moon Mountain, Congjiang County, Guizhou Province. In 1980, it was listed as a local pig breed in China; in 1982, it was compiled into the second episode of "Chinese Pig Breeds"; in 1993, it was listed as a national second-class protected breed by the Ministry of Agriculture. Fragrant pigs are short in size, relatively pure in genes, mellow in meat quality, and high in nutritional value. They are high-quality raw materials for processing high-quality meat products, and are also rare experimental animals in the field of life science research (Zhang Yi et al., 2006). [0003] Structural variations (SVs) usually refer to insertions, deletions, duplica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/156C12Q2600/172
Inventor 冉雪琴王嘉福喻昌燕牛熙
Owner GUIZHOU UNIV