A high-efficiency staining device for cell slides

Abstract

The invention relates to the technical filed of cell biology, in particular to a cell climbing slice efficient dyeing device. The cell climbing slice efficient dyeing device comprises a porous dyeing plate and a cell climbing slice assembly platform; the porous dyeing plate comprises a plurality of dyeing through hole and a sealing ring groove, a sealing ring is placed in the sealing ring groove, and four table board screw holes are formed in the four corners of the porous dyeing plate. The cell climbing slice assembly platform comprises a cell climbing slice containing table, a microscopic examination hole is formed in the center of the cell climbing slice containing table, and four table board screw holes are formed in the four corners of the cell climbing slice assembly platform. Different kinds of dyeing liquid or marking antibodies can be added into the dyeing through holes of the porous dyeing plate to mark corresponding areas on a cell climbing slice respectively, and after dyeing is completed, the dyeing condition can be observed through the microscopic examination hole. The cell climbing slice efficient dyeing device has the advantages of being simple in structure, low in cost and high in operability, the areas of the large-size cell climbing slice can be dyed respectively, therefore, the utilization rate of a cell sample is increased, the result detecting efficiency and accuracy are improved, the experiment efficiency can be effectively improved, and the experiment cost can be reduced.

Description

【Technical field】 [0001] The invention relates to the technical field of cell biology, in particular to a high-efficiency staining device for cell slides. 【Background technique】 [0002] In cell biology research, cell climbing method is often used to pretreat cells, and then perform immunofluorescence staining to observe cell life phenomena and study cell life activity rules. Under certain conditions, such as using a parallel plate flow chamber to stimulate cells with fluid shear stress, the size of the slide used is large (75mm×25mm), and the entire slide needs to be labeled with antibodies and washed during staining, which is likely to cause sticking during the operation. The parietal cells fall off, resulting in low experimental efficiency and high experimental costs. However, by using smaller-sized climbing sheets, the small-sized climbing sheets are easy to shake or wash away under the action of fluid shear force, which cannot solve this problem. [0003] At the same ...

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Problems solved by technology

[0004] At present, researchers lack an effective technical means for multi-staining labeling (non-co-staining) of large-scale cell slides

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