A three-dimensional cell scratch chip and its preparation method and application
A three-dimensional cell and chip technology, applied in the biological field, can solve the problems of low throughput, difference, poor repeatability, etc., and achieve the effect of improving consistency and improving experimental throughput.
Inactive Publication Date: 2019-02-15
INST OF ELECTRONICS CHINESE ACAD OF SCI +1
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Problems solved by technology
[0006] (1) Most of the existing scratch methods are two-dimensional methods, which cannot simulate the three-dimensional complex microenvironment of cells in vivo, resulting in a huge difference between the experimental model and the actual situation in vivo
[0007] (2) The existing three-dimensional scratch method cannot accurately control the spatial position and three-dimensional shape of three-dimensional cell clusters, and there are generally problems of poor repeatability and low throughput
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[0041] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments and with reference to the accompanying drawings.
[0042] The invention combines the chip technology with the scratch experiment, and designs a scratch chip with high throughput, good repeatability and the ability to realize three-dimensional cell migration. The structure of this chip is compatible with the traditional multi-well plate technology, and can be completely applied to the existing multi-well plate platform. The following will take a 96-well plate as an example to introduce the technical method of the present invention. Of course, the present invention is not limited thereto. It can also be an arbitrary array of N rows and M columns, wherein N is a natural number between 1-20, and M is A natural number between 1-30.
[0043] The specific implementation of this...
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Abstract
The invention provides a three-dimensional cell scratching chip and a preparation method and application thereof. The three-dimensional cell scratching chip comprises a substrate and small chamber arrays with opened upper sides and lower sides. The small chamber arrays are fixed to the substrate so that the substrate seals one side of each the small chamber array to form multiple small three-dimensional chambers with single opened sides. A metal layer is formed in the partial zone, located on one side of the substrate, of each small chamber, a self-assembly sulfur alcohol molecule layer is further formed on the metal layer, one end of each sulfur alcohol molecule in the self-assembly sulfur alcohol molecule layer is provided with first functional group reacted and absorbed with the corresponding metal layer, and the other end of each sulfur alcohol molecule is provided with a second functional group inhibiting cell absorption. The growth zones of cells are limited in the mode that the golden surfaces are modified by the self-assembly sulfur alcohol molecule layers containing the second functional groups so that three-dimensional cell clusters different in morphology can be cultured and can have clear scratching boundaries, and the consistency of three-dimensional scratching experiments can be improved.
Description
technical field [0001] The invention relates to the field of biotechnology, and further relates to a three-dimensional cell scratch chip and a preparation method of the three-dimensional cell scratch chip, and uses the chip to conduct research on cell migration. Background technique [0002] Cell migration is the basis of many important physiological activities and is highly controlled by cell physiological activities. Cell physiological activities are not only related to normal physiological processes such as embryonic development, tissue repair, wound healing, and angiogenesis, but also key links in pathological processes such as atherosclerosis, intimal hyperplasia, restenosis, and malignant tumor metastasis. Therefore, the study of cell migration has important theoretical significance and practical value for the understanding of physiological activities and the treatment of diseases. [0003] At present, the scratch method is one of the important methods to study cell m...
Claims
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IPC IPC(8): C12M1/00C12Q1/02
CPCC12M33/00G01N33/5029
Inventor 陈健卫元晨郝锐陈峰王军波陈德勇郭伟
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI