Preparation method and use method of novel adjuvant of oil emulsion inactivated vaccine

An application method and technology of oil emulsion, which is applied in the preparation and application field of new adjuvants of oil emulsion inactivated vaccines, can solve the problems that have not yet been reported in research reports, and achieve the goal of increasing antibody titer, speeding up express delivery, and eliminating immunosuppression Effect

Pending Publication Date: 2017-01-11
黑龙江省百洲生物工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The use of transfer factor solution in conjunction with attenuated vaccines has been proven to improve the immune effect of the vaccine, but there is no report on the use of transfer factor as an adjuvant for oil emulsion inactivated vaccines

Method used

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  • Preparation method and use method of novel adjuvant of oil emulsion inactivated vaccine
  • Preparation method and use method of novel adjuvant of oil emulsion inactivated vaccine
  • Preparation method and use method of novel adjuvant of oil emulsion inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Adjuvant Preparation

[0027] 1.1. The transfer factor dilution was filtered through a 0.22um polyethersulfone filter element, and samples were taken for sterility, polypeptide content, and ribose content testing. The sterility tests were all negative, the polypeptide content was 2.8mg / ml, and the ribose content was 58ug / ml.

[0028] 1.2. Add 4 parts of sterilized Tween-80 to 96 parts of transfer factor dilution, mix well, shake well at 37°C for 1 hour, take it out and put it at room temperature.

[0029] 1.3. Add 1 part of aluminum stearate to 94 parts of white oil, heat until the white oil becomes clear, add Siben-806 parts, and sterilize at 121°C for 30 minutes.

[0030] 1.4. Emulsify the oil phase and the water phase according to the ratio of 3:1, the emulsification temperature is 20°C, the emulsification speed is 3000rpm, and the emulsification is 20min.

[0031] 2. Routine vaccine preparation

[0032] 2.1. The Newcastle disease with HA titer of 1:1024 and the...

Embodiment example 1

[0052] Compare the quality effect of implementation case 1, control example 1, and control example 2

[0053] 1 shape

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Abstract

The invention discloses a preparation method of a novel adjuvant of an oil emulsion inactivated vaccine and a use method of the novel adjuvant in an oil emulsion inactivated vaccine and belongs to the technical field of preparation and use of the veterinary oil emulsion inactivated vaccine. The preparation method comprises taking a transfer factor diluent, carrying out filtration and degerming, adding tween-80 into the diluent according to a ratio to obtain a water phase, preparing an oil phase from white oil for injection and Span-80 according to a ratio, and carrying out emulsification on the water phase and the oil phase according to a ratio of 1: 2-1: 3 at a temperature of 15-35 DEG C at a rotation rate of 2500-3500rpm for 15-30min. The invention discloses a method for preparing an oil emulsion inactivated vaccine from the adjuvant. The method comprises carrying out emulsification mixing on the adjuvant and a routine vaccine according to a ratio of 1: 3 to 1: 4 to obtain a novel adjuvant vaccine. The novel adjuvant vaccine has immunization effects obviously better than those of the routine vaccine and has a short immunization blanking period and a uniform immunization antibody level higher than that of the routine vaccine.

Description

technical field [0001] The invention relates to the preparation of a new type of adjuvant for oil emulsion inactivated vaccines and the application method of the adjuvant in oil emulsion inactivated vaccines. The invention can realize the technical requirement of enhancing the immune effect of inactivated vaccines. Background technique [0002] my country's breeding environment is complicated, and animal diseases, pesticides, and veterinary drug residues have caused serious losses to the export of meat products. Only by reducing animal diseases, avoiding long-term use of antibiotics, and developing high-quality, pollution-free meat products can the sustainable and healthy development of my country's aquaculture industry be guaranteed. [0003] At present, in addition to safety measures, the prevention and control of animal infectious diseases mainly rely on vaccine immunization, especially through the immunization of oil emulsion vaccines. For oil emulsion inactivated vacci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K9/107A61P37/04
Inventor 李山峰张越尹鹏侯立立许翔杨艳坤马金花陈彦峰尹建丽
Owner 黑龙江省百洲生物工程有限公司
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