High-efficiency expanding propagation method for crossbred Chinese pennisetum

A hybrid Pennisetum and high-efficiency technology, applied in the field of plant tissue culture, can solve the problems of impure varieties, miscellaneous plants, affecting the quality and yield of planting Pennisetum, and achieve the effects of reducing production costs and stabilizing the propagation system.

Active Publication Date: 2017-01-18
贵州博安生物科技有限责任公司
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  • Abstract
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Problems solved by technology

At present, due to the impurity of hybrid pennisetum, there is a certain proportion of hybrid plants, which affects the quality and yield of planted pennisetum

Method used

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  • High-efficiency expanding propagation method for crossbred Chinese pennisetum
  • High-efficiency expanding propagation method for crossbred Chinese pennisetum

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Embodiment Construction

[0020] The present invention will be further introduced below in conjunction with the embodiments, but the present invention is not limited to the following embodiments. It is foreseeable that those skilled in the art may have various changes in the implementation situation in combination with the prior art.

[0021] An efficient propagation method for hybrid Pennisetum, its steps include:

[0022] 1) Selection and sterilization of explants

[0023] Take the young heart leaves of hybrid Pennisetum, scrub the surface with 75% ethanol, soak in 75% ethanol for 30s, rinse with sterile deionized water for 3 times, 0.1% HgCl 2 Soak for 5~8 minutes, rinse with sterile deionized water for 5 times, and absorb water on the surface of the material with sterile filter paper.

[0024] 2), callus induction

[0025] Cut the sterilized heart leaves into discs with a thickness of 0.2~0.5cm, and then connect them to callus induction medium at 26~28°C, incubate in the dark for 20~25 days. The medium comp...

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Abstract

The invention discloses a high-efficiency expanding propagation method for crossbred Chinese pennisetum, and belongs to the technical field of plant tissue culture. The high-efficiency expanding propagation method comprises the following steps: explant selection and sterilization, callus induction, embryonic callus induction and proliferation, multiple shoot induction, transplanting acclimatization, and the like. A stable and efficient expanding propagation system is established, the callus induction is up to 90%, the expanding propagation coefficient of the embryonic callus induction is as high as 20 times, the multiple shoot induction rate is up to 95%, and the acclimatization survival rate is up to 97%. Due to adoption of an ex vitro rooting mode, the production cost is greatly lowered, the method is applicable to industrialized seedling production, and assurance is provided or commercial planting of high-quality Chinese pennisetum.

Description

Technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for efficient propagation of hybrid pennisetum. Background technique [0002] Pennisetum is perennial, with thick roots, erect culms, clustered, densely pilose under the inflorescence, smooth leaf sheaths, flattened on both sides, and the upper culm is longer than the internodes. Flower in summer and bear fruit in autumn. It has the characteristics of high grass yield, strong stress resistance, good quality, and less pests and diseases. It is a high-quality green fodder for herbivorous livestock and poultry and an ideal crop for soil and water conservation. [0003] In recent years, with the continuous improvement of hybrid Pennisetum high-quality pulp, medium-density fiberboard production technology and biomass energy conversion technology, the demand for hybrid Pennisetum is increasing rapidly. At present, hybrid pennisetum has a certain percentage of hybrid pl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 刘红美梁大勇方小波石建龙
Owner 贵州博安生物科技有限责任公司
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