Diluent for in-vitro low temperature preservation of boar semen and preparation method thereof

Abstract

The invention discloses a diluent for in-vitro low temperature preservation of boar semen and a preparation method thereof. The diluent comprises following components: broad-spectrum antibiotic bacteriostatic agents of D-fructose, 2-amino-2-hydroxymethyl-1,3-propanediol, trisodium citrate, sodium bicarbonate, citric acid, disodium ethylene diamine tetraacetate, N-acetyl-cysteine, skim milk powder, yolk freeze-dried powder, amikacini sulfatis and the like. The diluent for the in-vitro low temperature preservation of the boar semen is suitable for preserving the fresh boar semen at low temperature (4-5 DEG C). The diluent significantly inhibits injury caused to sperm structure during the low temperature preservation process, maintains glucose metabolism and oxidative phosphorylation metabolism processes of boar sperm, effectively prolongs survival time of the boar sperm in the low temperature preservation, and significantly improves use efficiency of the high quality breeding boar semen and economic benefit of breeding.

Description

technical field [0001] The invention belongs to the technical field of animal reproduction, and in particular relates to a diluent for external low-temperature preservation of pig semen and a preparation method thereof. Background technique [0002] Countries such as Sweden, the United Kingdom, Canada, and the United States have established artificial insemination technology platforms and industrialized them in livestock animal breeding and actual reproduction production, with remarkable economic benefits. my country is a big pig raising and consumption country, with an annual production of more than 600 million pigs and about 65 million tons of pork. With the improvement of people's living standards, the per capita consumption of pork is increasing year by year, and the way of raising pigs is gradually changing from the traditional The way of rearing in courtyards has been transformed into factoryization, intensification and scale. Due to breeding and other factors in my co...

AI Technical Summary

Problems solved by technology

[0003] Frozen semen of livestock cattle and horses has been successfully used in breeding production, but the lack of corresponding technical standards for pig frozen semen breeding has not reached the popularization and promotion. The usage rate of pig frozen semen is less than 1%

However, pig semen stored at 4°C has not been used in actual production

Studies have confirmed that the integrity of the acrosome plasma membrane of sperm may be destroyed during low temperature storage at 4°C, causing the cytoskeleton to migrate to varying degrees, resulting in cells unable to withstand changes in osmotic pressure, sperm energy supply pathways are blocked, and intracellular ATP supply is insufficient, resulting in decreased viability (Zhao etc., Animal Science Journal, 2013, 84: 600-606); Secondly, during the storage of semen in vitro, too many unstable reactive oxygen ions (ROS) will be produced in the cells, which will cause ROS to deposit in sperm cells, causing cell Oxidative stress occurs, resulting in damage to sperm plasma membrane and acrosome, resulting in decreased motility (Roland et al., Theriogenology, 2006, 66: 1115-1123)

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