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Production method of animal feed enriched in carotenoid and probiotics

A technology of animal feed and carotene, which is applied in animal feed, animal feed, applications, etc., can solve the problems of easy infection of miscellaneous bacteria, little application research, and fermentation failure, so as to reduce the risk of miscellaneous bacterial infection, regulate blood pressure, anti-aging effect

Inactive Publication Date: 2017-01-25
深圳市中雁生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, Neurospora crassa does not produce acid during solid-state fermentation, and it is easy to infect miscellaneous bacteria, such as some spoilage bacteria, yeast and mold, etc., resulting in fermentation failure
In the past, it was known that Neurospora crassa is mainly red bread mold and spoilage bacteria of soybean products. It is a model organism that has been studied more in the field of microbiology, but there are few studies on its application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Use bean dregs with a water content of 60% as a solid medium, sterilize by steaming at 100°C for 50 minutes, cool to 20°C, and wait for inoculation. Neurospora crassa was inoculated in potato dextrose agar medium for activation and enrichment for 48 hours; Lactobacillus plantarum, Bifidobacterium, and Lactobacillus bulgaricus were inoculated in seed medium (peptone 10g / L, yeast extract 5g / L, NaCl 10g / L, pH 7.2-7.5), activated and enriched for 40 hours to obtain culture liquid. Add the mycelia or spores of Neurospora crassa to the culture medium at a weight ratio of 5%; meanwhile, the volume ratio of Lactobacillus plantarum, Bifidobacterium, and Lactobacillus bulgaricus is 1:1:1, according to the total bacterial liquid Volume (mL) / medium weight (g) 2% was added to the medium. Mix well and incubate at 25°C for 4 days.

Embodiment 2

[0018] Use bean dregs with a water content of 50% as a solid medium, sterilize by steaming at 100°C for 30 minutes, cool to 30°C, and wait for inoculation. Neurospora crassa was inoculated in potato dextrose agar medium for activation and enrichment for 24 hours; Lactobacillus plantarum, Bifidobacterium, Streptococcus thermophilus, Bacillus subtilis, and Enterococcus faecium were inoculated in seed medium (peptone 10g / L, yeast extract 5g / L, NaCl 10g / L, pH 7.2-7.5), activated and enriched for 24 hours to obtain a culture broth. Add the mycelia or spores of Neurospora crassa to the culture medium in a weight ratio of 10%; meanwhile, the volume ratio of Lactobacillus plantarum, Bifidobacterium, Streptococcus thermophilus, Bacillus subtilis, and Enterococcus faecium to 2 :2:1:1, according to the total volume of bacterial solution (mL) / medium weight (g) 10% added to the medium. Mix well and incubate at room temperature for 3 days.

Embodiment 3

[0020] Use bean dregs with a water content of 70% as a solid medium, sterilize by steaming at 100°C for 40 minutes, cool to 35°C, and wait for inoculation. Neurospora crassa was inoculated in potato dextrose agar medium for activation and enrichment for 48 hours; Lactobacillus plantarum, Bifidobacterium, Lactobacillus bulgaricus and Streptococcus thermophilus were inoculated in seed medium (peptone 10g / L, yeast extract 5g / L, NaCl 10g / L, pH 7.2-7.5), activated and enriched for 30h to obtain the culture broth. Add the mycelia or spores of Neurospora crassa to the culture medium at a weight ratio of 5%; meanwhile, the volume ratio of Lactobacillus plantarum, Bifidobacterium, Streptococcus thermophilus, Bacillus subtilis, and Enterococcus faecium to 1 :2:2:1, according to the total volume of bacterial solution (mL) / medium weight (g) 8% added to the medium. Mix well and incubate at room temperature for 2 days.

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Abstract

The invention relates to a production method of animal feed enriched in carotenoid and probiotics. The production method includes the steps of i, preparing bean dregs with water content of 40%-70% as solid culture medium, steaming and sterilizing for 20-50 min at 100 DEG C, and cooling to 20-40 DEG C; waiting for inoculating; ii, inoculating neurospora crassa to a potato dextrose agar culture medium and activating and enrichment-culturing for 24-48 h, collecting mycelium and conidium of neurospora crassa; iii, respectively inoculating probiotics culture to a seed medium; performing the enrichment-culturing for 10-40 h, and obtaining probiotics culturing bacterial liquid; iv, adding mycelium and conidium of neurospora crassa to a solid culture medium according to the weight ratio of 2%-15%, and adding the probiotics culturing bacterial liquid to the solid culture base according to the ratio of 1%-10% between the total volume of bacterium (mL) and weight of culture medium (g) in the solid culture medium, and evenly mixing, and culturing for 1d-4d under the condition of 20-40 DEG C. The feed prepared by the method of the invention is rich in carotenoid and probiotics, and good for the health of animals after eating.

Description

technical field [0001] The invention relates to the field of feed production, in particular to a production method for animal feed enriched with carotenoids and probiotics. Background technique [0002] Neurospora crassa (Neurospora crassa) belongs to the fungus phylum, Euascomycetes subclass, Staghornomycetes, Faecaliaceae, is a common fungus belonging to the Neurospora genus, it is organized by the US Food and Drug Administration FDA It is considered as one of the few safe edible fungi. Neurospora crassa is the dominant growth bacterium in the solid-state fermentation of soybean dregs as raw material. During the fermentation process, a large number of conidia can be produced, and the conidia are rich in carotenoids, mainly β-carotene. Neurospora crassa has strong fiber decomposition ability, and can degrade crude fiber into oligosaccharides or monosaccharides. Therefore, Neurospora crassa has a good application prospect. [0003] However, Neurospora crassa does not prod...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/18A23K10/37
CPCY02P60/87
Inventor 谢汝朋
Owner 深圳市中雁生物工程有限公司
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