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Multi-tumor-marker detecting platform and method based on SERS detecting technology and micro-fluidic chip

A microfluidic chip and tumor marker technology, applied in the fields of spectroscopy and biological analysis, can solve the problems of high mortality in breast cancer patients, and achieve the effects of improving sensitivity, saving costs, and high detection sensitivity

Inactive Publication Date: 2017-01-25
SOUTHEAST UNIV
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Problems solved by technology

[0002] In recent years, despite the increasing understanding of the evolution of breast cancer and the continuous improvement of medical methods, the mortality rate of breast cancer patients is still very high

Method used

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  • Multi-tumor-marker detecting platform and method based on SERS detecting technology and micro-fluidic chip
  • Multi-tumor-marker detecting platform and method based on SERS detecting technology and micro-fluidic chip

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Embodiment

[0076] Such as figure 1 and as figure 2 Shown, a kind of platform detection process that can simultaneously detect multiple tumor markers and can improve detection sensitivity based on SERS detection technology and microfluidic chip of the present invention comprises the following steps:

[0077] 1) Preparation of silver nanoparticles, at first, 390ml deionized water and 0.072g AgNO 3 Heat to boiling at 130°C under stirring, then add 10ml of 1% sodium citrate, continue heating until the solution turns yellow-green, stop heating, let the solution cool at room temperature, and make silver colloid for later use.

[0078] 2) Preparation of SERS probe: Take 6ml of silver colloid and centrifuge at 11000rpm for 30 minutes in a centrifuge, remove the supernatant and add 4ml of particle-free water. Then, 40 μl of 10 mmol 4MBA was added to 4 ml of the silver colloid solution and allowed to mix for at least two hours under stirring. Finally, 1.5 ml of the mixed solution was centrifug...

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Abstract

The invention discloses a multi-tumor-marker detecting platform based on a SERS detecting technology and a micro-fluidic chip. A SERS substrate is formed in a micro-fluidic channel at first to improve detection sensitivity, and then a typical sandwich structure is formed on the SERS substrate to detect whether a to-be-detected reagent contains specific tumor markers or not. The substrate is connected with a specific capturing antibody by a PDMS chip at first, and then a placing position of a new PDMS chip is perpendicular to a previous placing position to achieve the effect of simultaneously detecting multiple tumor markers in an intersection region. A SERS immunoprobe which is connected with a second antibody and a Raman reporting molecule to achieve the effect of qualitatively and quantitatively detecting the tumor marks in the to-be-detected reagent, the second antibody can amplify signals, and therefore, detection sensitivity can be improved further. The whole platform can simultaneously detect the multiple tumor markers qualitatively and quantitatively, and provides favorable technical support for early diagnosis of cancer of cancerous persons and increasing of survival rate.

Description

technical field [0001] The invention belongs to the technical field of spectroscopy and biological analysis, and in particular relates to a detection platform and method for multiple tumor markers based on SERS detection technology and a microfluidic chip. Background technique [0002] In recent years, despite the increasing understanding of the evolution of breast cancer and the continuous improvement of medical methods, the mortality rate of breast cancer patients is still very high. One of the reasons is that breast cancer patients are diagnosed with breast cancer relatively late, so it is very important to improve the accuracy of early diagnosis of breast cancer patients, which can improve the survival rate of breast cancer patients. [0003] So far, a large number of experiments have proved that tumor markers have great potential for early diagnosis and monitoring of cancer. However, it is often inaccurate to detect only one tumor marker to determine whether a patient ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/543G01N21/65B01L3/00
CPCG01N33/57484B01L3/5027B01L2200/10G01N21/658G01N33/54306G01N33/57415
Inventor 王著元郑志华崔一平伍磊宗慎飞
Owner SOUTHEAST UNIV
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