Quantitative detection card, preparation method thereof and detection method

Abstract

The invention relates to a quantitative detection card, a preparation method thereof and a detection method. The quantitative detection card comprises a bottom card and a surface card. The bottom card is provided with a sample application region, a bonding region, a micro-channel, a time valve, a detection region and a waste liquid region. The sample application region is connected with the bonding region. The sample application region and the bonding region are located at one end of the micro-channel. The detection region is connected with the other end of the micro-channel through the time valve. The surface card is provided with a sample application hole and an observation hole. The position of the sample application hole is corresponding to the position of the sample application region on the bottom card. Based on the immunofluorescence technique, the variation of an antibody at different time is detected. After that, the detection result is converted into the concentration of anti-17 alpha-hydroxyprogesterone antibody, the concentration of anti-luteinizing hormone antibody and the concentration of anti-follicle-stimulating hormone antibody in a to-be-detected sample according to a variation curve, so that the quantitative detection level is realized. The quantitative detection card, the preparation method thereof and the detection method have the advantages of simple operation, short detection time, simple storage, easy popularization and easy usage.

Description

technical field [0001] The invention relates to the technical field of combining microfluidic technology and immunofluorescence microsphere immunochromatography technology, in particular to a quantitative detection card and its preparation method and detection method. Background technique [0002] 17α-Hydroxyprogesterone (17α-OH-Progesterone, 17α-OHP) is an endogenous progesterone produced during the synthesis of adrenal glucocorticoids and sex steroids, and is mainly derived from the adrenal cortex during the neonatal period. In the cholesterol metabolism pathway, 17α-OHP is converted into 11-deoxycortisol under the catalysis of 21-hydroxylase, and finally generates cortisol. 21-hydroxylase, encoded by CPY21A2, is a cytochrome enzyme located in the endoplasmic reticulum of the adrenal cortex. 21-hydroxylase deficiency hinders the synthesis of cortisol, promotes the increase of adrenocorticotropic hormone through negative feedback, leads to adrenal cortical hyperplasia, and...

AI Technical Summary

Problems solved by technology

However, at present, there is no report on the normal reference value of human sex hormones measured by electrochemiluminescence method in China. The range of foreign reference values ​​is too large, which lacks guiding significance for clinical application.

These methods are all qualitative or semi-quantitative detection methods, and cannot detect the content levels of 17α-hydroxyprogesterone, hLH and hFSH in blood or urine

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