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A kind of method for in vitro regeneration of Begonia waschii

An in vitro regeneration and begonia technology, applied in the field of plant tissue culture, can solve the problems of difficulty in planting a single plant, inability to reproduce in large quantities, and difficulty in surviving cuttings of begonias

Inactive Publication Date: 2019-04-09
SHANGHAI CHENSHAN BOTANICAL GARDEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the seeds of Begonia varssonii are small, only half the size of the seeds of Arabidopsis thaliana, difficult to distinguish with the naked eye, difficult to plant a single plant, and, in the greenhouse or under natural conditions, the cuttings of begonia varssonii are not easy to survive and cannot be reproduced in large quantities
At present, there is no relevant report on the tissue culture of Begonia varsundii. Therefore, it is necessary to establish a method for the in vitro culture and regeneration system of Begonia varsundii, which can be used for large-scale rapid propagation, as well as for future molecular breeding and Molecular biology studies of Begonias establish a foundation

Method used

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  • A kind of method for in vitro regeneration of Begonia waschii
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  • A kind of method for in vitro regeneration of Begonia waschii

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Embodiment 1

[0045] Embodiment 1 A kind of in vitro regeneration method of Begonia waschii comprises the following steps:

[0046] 1) Germination culture

[0047] Sterilize the seeds of Begonia waschii, suspend them with sterilized 0.1% low-melting point agarose, sow them on the germination medium, and carry out light culture. The culture temperature is 20-25°C, the light time is 16 hours / day, and the seeds grow for 10 ~15 days after germination (see figure 1 ), the germination rate is almost 100%, and seedlings are formed. When a small amount of roots appear at the base of the seedlings, the seedlings are divided into single plants and transplanted, and continue to be cultivated on the germination medium to grow young leaves until the leaves are launched (see figure 2 ).

[0048] Wherein, the germination medium comprises: MS medium 2.2g / L, sucrose 20g / L and plant gel 4g / L, pH is 5.8-6.2;

[0049]2) Induction culture

[0050] Cut the young leaves of step 1) into an area of ​​0.5-1cm ...

Embodiment 2

[0059] A kind of in vitro regeneration method of Begonia waschii, comprising the steps of:

[0060] 1) Induction culture

[0061] The leaves of Begonia waschii grown in the wild were used as explants and cut into an area of ​​0.5-1 cm 2 See Table 2 for the disinfection treatment conditions.

[0062] Table 2

[0063] Numbering

Disinfection method

Number of explants

explant survival rate

The present invention 1

0.1% HgCl 2 ,8 minutes

20

69％

Invention 2

0.1% HgCl 2 , 4 minutes

20

69％

Comparative example 1

70% alcohol for 30 seconds, 0.1% HgCl 2 8 minutes

20

0％

[0064] As can be seen from Table 2, after 70% alcohol treatment for 30 seconds, the explants processed with 0.1% HgCl are all dead, while the present invention uses 0.1% HgCl solution to sterilize the leaves for 4 ~ 8 minutes can ensure that the explants are thoroughly sterilized and have a higher survival rate.

[0065] Inoculate the ...

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Abstract

The invention discloses an in vitro regeneration method of Begonia wallichiana Lehm. The in vitro regeneration method comprises following steps: germination culturing of Begonia wallichiana Lehm seeds, induction culturing, and rooting culturing; and study on culture mediums is carried out. According to the in vitro regeneration method, an appropriate plant growth regulator and an appropriate concentration are selected based on the growth characteristic of Begonia wallichiana Lehm, leaves of Begonia wallichiana Lehm can be induced into adventitious bud with pertinency, induction of root growth is realized, and at last complete plants can be obtained via growth of test-tube plantlets, and survival rate of transplanting is 95% or higher. The in vitro regeneration method is capable of realizing rapid high-efficiency breeding of Begonia wallichiana Lehm, and excellent foundation is provided for reasonable utilization of Begonia wallichiana Lehm in the future.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for in vitro regeneration of Begonia valssonii. Background technique [0002] Begonia wallichiana Lehm (Begonia wallichiana Lehm) is a perennial herbaceous plant belonging to the Begoniaceae family (Begoniaceae), native to Mexico and other places in North America. Begonia waschii grows fast, can bloom in all seasons, monoecious, white flowers; at the same time, it has strong resistance, the surface of the leaves is ciliated, and it is not easy to breed diseases and insect pests. It is a potential candidate species for becoming a model plant , is also a good home ornamental plant. [0003] However, the seeds of Begonia varssonii are small, only half the size of the seeds of Arabidopsis thaliana, difficult to distinguish with the naked eye, difficult to plant a single plant, and, in the greenhouse or under natural conditions, the cuttings of Begonia varsson...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 田代科张大生陈青赵喜双付乃峰刘青青陈蒙娇吴少华
Owner SHANGHAI CHENSHAN BOTANICAL GARDEN