SNP mark screening method for monitoring breed conservation effect of poultry, application thereof to chicken breed conservation and authentication method of SNP mark

A screening method and identification method technology, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of limited number of molecular markers, insufficient monitoring efficiency, and lack of information on genome genetic variation, and achieve good application prospects. , the effect of improving scientificity and accuracy

Pending Publication Date: 2017-02-22
JIANGSU INST OF POULTRY SCI
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Problems solved by technology

The number of these molecular markers is very limited (for example, there are only about 30 microsatellite markers commonly used for the analysis of chicken genetic diversity, and these markers are not fully ap...

Method used

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  • SNP mark screening method for monitoring breed conservation effect of poultry, application thereof to chicken breed conservation and authentication method of SNP mark
  • SNP mark screening method for monitoring breed conservation effect of poultry, application thereof to chicken breed conservation and authentication method of SNP mark
  • SNP mark screening method for monitoring breed conservation effect of poultry, application thereof to chicken breed conservation and authentication method of SNP mark

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Embodiment Construction

[0045] The purpose of the present invention is to identify a high-density SNP molecular marker suitable for monitoring chicken breed conservation effect and its application. The technical solution of the present invention is: use the simplified genome RAD-seq sequencing technology to sequence different local chicken breeds, and identify consistent SNP sites that are concentratedly distributed on the genomes of different chicken breeds and have relatively rich genetic diversity through sequence comparison . By counting the allele frequencies of SNP sites, calculating genetic parameters such as population heterozygosity H, polymorphic information content PIC, etc., monitoring the conservation effect according to the changes of genetic parameters in the process of the conservation population passing from generation to generation. The materials and methods used and the results obtained are as follows:

[0046] 1 Materials and methods

[0047] 1.1 Experimental materials

[0048]...

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Abstract

The invention relates to a high-density SNP molecular mark screening method for monitoring the breed conservation effect of poultry, application there to chicken breed conservation and an authentication method of an SNP molecular mark. Multiple breeds of poultry of the same kind are selected, multiple individuals are selected for each breed of poultry, and SNP screening includes the following steps that 1, all the breeds of poultry are shared, and each SNP site at least has two alleles in any breed; 2, distribution is relatively concentrated on a gene group, and the number of SNP sites within the interval of the gene group 1M is larger than 90; 3, genetic diversity is abundant, and the minimum allele frequency of each SNP site in any breed is larger than 0.1, and the SNP sites meeting the requirements of the step1, step2 and step3 are obtained through screening. A simplified gene group RAD-seq sequencing technology is utilized for sequencing chicken breeds at different places, and the uniform SNP sites which are distributed on different chicken gene groups in a concentrated mode and have abundant genetic diversity are authenticated through sequence comparison.

Description

technical field [0001] The invention relates to a high-density SNP molecular marker screening method for monitoring poultry breed conservation effect and its application in chicken breed conservation, and an identification method of SNP molecular marker, belonging to the fields of population genetics and molecular biology. Background technique [0002] my country is one of the countries with the richest chicken breed resources in the world. There are 107 local chicken breed resources. Protecting these precious genetic resources is of great significance to the sustainable development of my country's poultry industry and even the world's poultry industry. [0003] Due to the special oviparous reproduction mode of poultry, live species preservation is the most important form of chicken breed resource preservation. Maintain a certain number of conservation populations, and effectively slow down the increase in the population's inbreeding coefficient and maintain its genetic dive...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q1/6888C12Q2600/156C12Q2535/122C12Q2539/10
Inventor 韩威苏一军李国辉王洪志朱云芬盛中伟张会永殷建玫邹剑敏
Owner JIANGSU INST OF POULTRY SCI
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