Structural variation SV200 molecular marker for identifying local pig breeds

A technology of structural variation and molecular markers, which is applied in the field of structural variation molecular markers to identify local pig breeds and Large White pigs, can solve the problems of unclear function of SV200, and achieve the effect of simple operation, accurate and reliable method

Active Publication Date: 2017-02-22
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Unknown function of structural vari...

Method used

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  • Structural variation SV200 molecular marker for identifying local pig breeds
  • Structural variation SV200 molecular marker for identifying local pig breeds
  • Structural variation SV200 molecular marker for identifying local pig breeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 The detection technology of porcine structural variation SV200, the steps are as follows:

[0029] 1. Genomic DNA extraction

[0030] The present invention adopts the blood / cell / tissue genomic DNA extraction kit (spin column type) produced by Tiangen Biochemical Technology (Beijing) Co., Ltd. to extract genomic DNA, and the specific method is as follows:

[0031] 1) Processing materials

[0032] a. If the material is blood, take 200 μL of fresh, frozen or blood with various anticoagulants directly, and add buffer GA to make up for less than 200 μL.

[0033] b. If the material is tissue, cut up 50 mg tissue sample, add 200 μL buffer GA, and shake until completely suspended.

[0034] 2) Add 20 μL of Proteinase K solution and mix well.

[0035] a. When extracting the blood genome, just add Proteinase K solution and mix well, then proceed to the next step;

[0036] b. When extracting the tissue genome, add Proteinase K solution and mix well, bathe in water at ...

Embodiment 2

[0052] Example 2 Distribution and Detection Application of Structural Variation SV200 Genotype in Various Pig Breeds

[0053] According to the method of embodiment 1, test material is as shown in table 1, is candidate structural variation with chr 6:17953135-17953289, named after SV200, utilizes PCR technology to detect the genotype of structural variation SV200 in 6 pig breeds (table 2 and figure 2 ), and the χ2 test in SPSS v20.0 software was used to analyze the gene frequency of structural variation SV200. The results show that the structural variation SV200 of local pig breeds is dominated by the "deletion" gene (D), and the Large White pig is dominated by the "normal" A gene, and the gene frequency difference between the local pig breeds and the Large White pig population is extremely significant (P< 0.01).

[0054] Table 1 Statistical table of test materials and sources

[0055]

[0056]

[0057] Table 2 Genotypes and gene frequencies of structural variant SV200...

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Abstract

The invention discloses a structural variation SV200 molecular marker for identifying local pig breeds. The structural variation SV200 molecular marker is characterized in that the structural variation SV200 is positioned at chr 6: 17953135-17953289 of a pig reference genome Sscrofa 10.2; a deletion fragment has the length of 155bp and is positioned in an intron 5 of an MMP-15 gene; the deletion gene of the structural variation SV200 is defined as D, a normal non-deletion gene is defined as A, and the frequency of a D gene in 5 local pig breeds is significantly higher than a corresponding value of a big white pig. The structural variation SV200 molecular marker can be used as the molecular marker for identifying the local pig breeds.

Description

technical field [0001] The invention relates to the field of livestock molecular biology detection, in particular to a structural variation molecular marker technology for identifying local pig breeds and large white pigs. Background technique [0002] Since wild boars were domesticated, factors such as living environment and artificial selection have created a variety of domestic pig breeds, especially local pig breeds have great differences in body appearance, behavioral characteristics, growth and reproductive performance, etc. (Wang Yanan, 2015) . Guizhou local pig breeds are robust in physique, pure in genes, tender in meat quality, strong in stress resistance, resistant to rough feeding (Jiang Huimei et al., 2012), and rich in genetic diversity. , It has obvious advantages in terms of lean meat rate and growth rate. The advantages of the two types of pig breeds will be selectively utilized in practical production according to human needs, which is the basis for new b...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 冉雪琴王嘉福喻昌燕牛熙
Owner GUIZHOU UNIV
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