Automatic dosing and mixing method and device for detecting nitrite nitrogen content

Abstract

The invention discloses an automatic chemical feeding and mixing method and device for detecting the nitrite nitrogen content. The method comprises the steps of injecting distilled water in a distilled water bottle in to a cuvette through a multiported valve, a micro-injection pump and a scaling liquid two-station two-way electromagnetic valve; collecting blank-value voltage values through a light emission device and a receiving device; injecting an external water sample into the cuvette; adding two kinds of chemicals of 4-aminobenzenesul fonamide and hydrochloric acid solution and N-(1-naphthyl)-ethylene diamine dihydrochloride solution into the cuvette; controlling four vibration base electromagnets and coils in a vibration base to be electrified; sequentially electrifying four vibration slider electromagnets and coils; mixing the chemicals and a water sample in the cuvette; collecting voltage values through the light emission device and the receiving device; calculating out an absorbance value of mixed solution according to a formula. The detection efficiency of the nitrite nitrogen content in water is improved through automatic sampling, chemical addition and detection.

Description

technical field [0001] The invention relates to the field of detection of aquaculture water quality environment, in particular to the detection technology of nitrite nitrogen content, in particular to the automatic dosing and mixing technology for detection of nitrite nitrogen. Background technique [0002] In the process of aquaculture, excessive stocking and concentrated feeding produce a large amount of residual bait, metabolites of fish and shrimp, and dead animal and plant corpses, which are spoiled and fermented under the action of heterotrophic bacteria, which often leads to nitrogen content. It accumulates in a large amount in the aquaculture water body, and this different form of nitrogen can be converted into nitrite nitrogen through the action of microorganisms; the nitrite nitrogen in the water quality of the pond mainly enters the blood from the gills through the respiration of fish, which is different from the Hemoglobin undergoes a redox reaction to produce me...

AI Technical Summary

Problems solved by technology

[0002] In the process of aquaculture, excessive stocking and concentrated feeding produce a large amount of residual bait, metabolites of fish and shrimp, and dead animal and plant corpses, which are spoiled and fermented under the action of heterotrophic bacteria, which often leads to nitrogen content. It accumulates in a large amount in the aquaculture water body, and this different form of nitrogen can be converted into nitrite nitrogen through the action of microorganisms; the nitrite nitrogen in the water quality of the pond mainly enters the blood from the gills through the respiration of fish, which is different from the Hemoglobin undergoes a redox reaction to produce methemoglobin without oxygen-carrying capacity, resulting in decreased hemoglobin activity or functional anemia, and loses the function of carrying oxygen, resulting in hypoxia, poor vitality, reduced food intake, susceptibility to germs, and even death

[0003] At present, the methods for determining nitrite nitrogen mainly include diazo coupling method, catalytic photometry, nitrosation method, ion chromatography, sensor method, etc. Among these detection methods, the most widely used detection method is diazo coupling method. Other methods are limited in terms of cost, application range, and detection stability, and the diazo coupling method needs to add reagents to the water sample for sufficient reaction before the detection of nitrite nitrogen content in water quality can be completed.

At present, nitrite nitrogen detection instruments mainly include hand-held nitrite nitrogen detectors, online nitrite nitrogen detectors, nitrite nitrogen detection test papers, etc., and these instruments all use the double azo method; The dosing of the nitrite nitrogen detector and the nitrite nitrogen test paper is manually added dropwise, and after adding the medicine, it needs to be shaken manually, then put the cuvette into the cuvette, and let it stand for twenty The online nitrite nitrogen detector adopts the flow injection method for dosing and sampling, and the detection is completed through the cooperation of the peristaltic pump and the multi-way valve, and the colorimetric cuvette for the reaction between the medicament and the water sample is It is designed with a fixed structure, that is to say, the online nitrite nitrogen detection instrument can only complete the process of dosing and has no function of processing the mixture after adding the medicine.

Therefore, whether it is a hand-held or online nitrite nitrogen detector, it is necessary to add reagents to the water sample to make it fully react before detection, and the content of nitrite nitrogen in the water quality is a small amount, because the chromogenic agent is in the water The diffusion in the sample is very slow, and the reaction time is long, which leads to a longer detection cycle, and the approximate detection cycle is about 20 minutes.

In summary, the current detection of nitrite nitrogen has the disadvantages of manual shaking, fixed cuvette, and long detection cycle.

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