A method for the detection of triazophos in sediment by ultra-high performance liquid chromatography mass spectrometry

Abstract

The invention discloses an ultra-high performance liquid chromatography-mass spectrometry detection method of triazophos in bottom mud sediment. The method comprises steps as follows: (1) vortex oscillation and ultrasonic solvent extraction; (2) dispersive solid-phase extraction and solid-phase extraction column enrichment and purification: graphitized carbon black and N-propyl ethylenediamine are added to an extracting solution, vortex oscillation and centrifugation are performed, the mixture is treated with anhydrous sodium sulfate, rotary evaporation is performed until the mixture is dried, a to-be-detected liquid is obtained after n-hexane is added for washing and dissolution, the to-be-detected liquid is added to a magnesiumtrisilicate solid-phase extraction column and eluted with dichloromethane, and an eluant is collected; (3) ultra-high performance liquid chromatography-mass spectrometry detection: the eluant is taken for liquid chromatography-mass spectrometry detection, then, a standard working curve is drawn, quantification is performed with an external standard method, and the concentration of triazophos is calculated according to the peak area. The method is convenient to operate, better in reproducibility, accurate in qualitative detection and higher in recovery rate and can be used for rapidly analyzing triazophos in the bottom mud sediment.

Description

technical field [0001] The invention relates to the technical field of fishery environment monitoring, in particular to an ultra-high performance liquid chromatography mass spectrometry detection method for triazophos in sediment sediment. Background technique [0002] Triazophos is a broad-spectrum organophosphorus insecticide, acaricide, and nematocide, mainly used for the control of lepidopteran pests, harmful mites, fly larvae and insects on fruit trees, cotton, and food crops. Subterranean pests, etc., have strong contact, stomach poisoning and systemic effects. With the prohibition of most organochlorine and organophosphorus highly toxic pesticides, the demand for triazophos is increasing because it can be degraded relatively quickly in the environment, and it is difficult to be completely replaced by other pesticides in a short period of time. Shi Lili studied the degradation characteristics in three different types of soil, showing that the degradation half-lives ar...

AI Technical Summary

Problems solved by technology

The current detection and analysis of triazophos is generally based on GC and GC-MS, but its sensitivity to triazophos is about 10ppb, which sometimes cannot meet the analysis requirements of trace triazophos in sediment sediments. The sample matrix of sediment sediments is complex. If the impurities are not removed well during the sample pretreatment process, it may directly interfere with the measurement results of the instrument, or even damage the instrument.

At present, there is no domestic method for the detection of triazophos in sediment sediments by ultra-high liquid chromatography mass spectrometry

Images