Identification method for parched white peony root in Shiquan Dabu wine
A technology of Shiquan Dabu wine and fried white peony, which is applied in the field of traditional Chinese medicine detection to achieve good reproducibility, ensure product quality, and scientifically and effectively identify the effect
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Embodiment 1
[0043] The identification method of fried white peony in Shiquan Dabu wine includes the following steps:
[0044] (1) Preparation of the test solution: Take 25 mL of this product, add 25 mL of n-butanol and shake to extract, obtain n-butanol liquid, evaporate to dryness, add 2 mL of methanol to the residue to dissolve it, and use it as the test solution;
[0045] (2) Preparation of reference substance solution: take paeoniflorin reference substance, add methanol to make a solution containing 1 mg of paeoniflorin reference substance per 1 mL, as the reference substance solution;
[0046] (3) Identification: According to the "Chinese Pharmacopoeia" 2015 edition four general rules 0502 thin-layer chromatography test, absorb 9 μL of the reference substance solution and 9 μL of the test solution, respectively spot on the same silica gel G thin-layer plate, with chloroform-acetic acid Ethyl ester-methanol-formic acid=35︰3︰8︰0.1 is the developer, develop, take out, dry, spray with 5%...
Embodiment 2
[0048] The identification method of fried white peony in Shiquan Dabu wine includes the following steps:
[0049] (1) Preparation of the test solution: Take 35 mL of this product, add 45 mL of n-butanol and shake to extract, obtain n-butanol liquid, evaporate to dryness, add 3 mL of methanol to the residue to dissolve it, and use it as the test solution;
[0050] (2) Preparation of reference substance solution: take paeoniflorin reference substance, add methanol to make a solution containing 1 mg of paeoniflorin reference substance per 1 mL, as the reference substance solution;
[0051] (3) Identification: According to the "Chinese Pharmacopoeia" 2015 edition four general rules 0502 thin-layer chromatography test, absorb 12 μL of the reference substance solution and 12 μL of the test solution, respectively spot on the same silica gel G thin-layer plate, with chloroform-acetic acid Ethyl ester-methanol-formic acid=45:8:15:0.3 is the developer, develop, take out, dry, spray with...
Embodiment 3
[0053] The identification method of fried white peony in Shiquan Dabu wine includes the following steps:
[0054] (1) Preparation of the test solution: take 20mL of this product, add 30mL of n-butanol and shake to extract to obtain n-butanol liquid, evaporate to dryness, add 1mL of methanol to the residue to dissolve it, and use it as the test solution;
[0055] (2) Preparation of reference substance solution: take paeoniflorin reference substance, add methanol to make a solution containing 1 mg of paeoniflorin reference substance per 1 mL, as the reference substance solution;
[0056] (3) Identification: According to the "Chinese Pharmacopoeia" 2015 edition four general rules 0502 thin-layer chromatography test, absorb 10 μL of the reference substance solution and 10 μL of the test solution, respectively spot on the same silica gel G thin-layer plate, and use chloroform-acetic acid Ethyl ester-methanol-formic acid=40:5:10:0.2 is the developer, develop, take out, dry, spray wi...
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