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Large Daphnia Flowing Water Toxicity Test Device

A toxicity test, flow-through technology, applied in the direction of measuring devices, biological tests, material inspection products, etc., can solve the problems of long-term research needs of water ecotoxicity that are difficult to meet the test requirements, difficulties in chemical substance testing and scientific research, and low efficiency. Achieve the effects of avoiding escape and mechanical damage, facilitating test operation, and reducing environmental hazards

Active Publication Date: 2018-09-07
NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex, fine, and special technical requirements mentioned above, there is no equipment or device that meets the requirements on the market at present. The laboratories that have carried out large-scale daphnia toxicity research in my country can only use static and semi-static tests manually.
This method is not only inefficient, but also difficult to meet the testing requirements of unstable "difficult substances" and the long-term research requirements of chronic toxicity of chemical substances and environmental endocrine disrupting aquatic ecotoxicity
This brings greater difficulties and errors to chemical substance testing and scientific research

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0034] Such as figure 1 In the Daphnia daphnia running water toxicity test device shown, the symbols or letters are indicated as follows: 1 is the medium storage barrel, used to store Daphnia magna culture medium; 2 is the solution preparation room for the solution preparation system, and the test solutions with different concentrations are in the The preparation is completed here, and the solution preparation system includes reagent bottles (level I mother solution bottle, level II mother solution bottle, solvent bottle, cosolvent control preparation bottle, blank control preparation bottle, test solution preparation bottle), magnetic stirrer, peristaltic pump, PLC controller; 3 is the solution temporary storage room for the solution temporary storage system, where the prepared test solution is temporarily stored and fully homogenized. The solution temporary storage system includes reagent bottles (cosolvent control temporary storage bottle, blank control Temporary storage b...

Embodiment 2

[0061] Assuming that a large-scale daphnia running water toxicity test is required, the test concentration is designed as: blank control, cosolvent control, 0.1mg / L, 0.2mg / L, 0.4mg / L, 0.8mg / L, 1.6mg / L, the concentration The interval factor is 2. The solution update frequency is required to be no less than 12 hours (that is, all solutions in the test tank must be updated once within 12 hours). Environmental condition control must meet the requirements of Chemical Testing Guidelines (HJ / 153-2004) and "Chemical Testing Methods" (Second Edition, China Environmental Publishing House). The implementation of the present invention is as follows:

[0062] (1) Turn on the constant temperature water bath, set the temperature at 20±1°C, turn on the light and set the photoperiod as 16h (light): 8h (dark).

[0063] (2) The procedure for setting the peristaltic pump is as follows:

[0064] Peristaltic pump 1

[0065] Program 1 (P1P1): channel 1 flow rate 0.50mL / min, forward run 40min

[0...

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Abstract

The invention relates to a running water type toxicity test device for daphnia magna. The device comprises a solution preparation system, a temporary solution storage system, a toxicity exposure system, an automatic solution circulation and supplementation system and a waste liquid treatment system, wherein the solution preparation system is used for preparing a tested solution with the specific concentration for a daphnia magna toxicity test; the temporary solution storage system provides conditions for homogenization and temporary storage of the tested solution; the toxicity exposure system is used for providing appropriate space, temperatures and illumination for exposure of the daphnia magna in a test solution; the automatic solution circulation and supplementation system is connected between the solution preparation system and the temporary solution storage system and between the temporary solution storage system and the toxicity exposure system and can automatically supplement a solution from the preparation system to the temporary solution storage system and convey the solution from the temporary solution storage system to a toxicity test system; the waste liquid treatment system provides purification functions including filtration, adsorption and the like for a waste liquid produced by the toxicity exposure system.

Description

technical field [0001] The invention belongs to the field of ecotoxicology experiments, and in particular relates to a large daphnia running water toxicity test device. Background technique [0002] Daphnia large ( Daphnia magna Straus) belongs to Arthropoda, Crustacea, and Cladocera, is a common planktonic crustacean, and is an indispensable component in the freshwater food chain. Because of its short life cycle, fast growth, high reproductive capacity, easy cultivation, and sensitivity to water environment stress, it has become a model organism widely used in laboratories at home and abroad. Plays an important role in testing. [0003] At present, many international organizations and countries such as OECD, European Union, the United States, Japan, South Korea and my country have formed systematic standard methods for toxicological testing of Daphnia magna. In order to ensure the scientificity, validity and comparability of the test results, most of these methods requir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50
CPCG01N33/5014G01N33/5085G01N2333/43508
Inventor 王蕾刘济宁汪贞范德玲石利利
Owner NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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