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liquid stirring method

A stirring method and liquid volume technology, which can be used in mixing methods, mixer accessories, chemical instruments and methods, etc., and can solve the problems of repeating a certain number of times.

Active Publication Date: 2018-12-14
HITACHI HIGH-TECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Stirring by inhalation and exhalation is sometimes performed once each, but sometimes it is repeated a certain number of times

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0021] figure 1 It is a diagram showing the outline of an automatic analyzer to which the liquid stirring method according to the present invention is applied.

[0022] figure 1 Among them, the automatic analysis device 200 roughly includes: a rack 202 that accommodates a plurality of sample containers 201 containing biological samples such as blood and urine (hereinafter referred to as specimens); a rack transfer line 203 for transferring the rack 202; a reagent container tray 205, the reagent container tray 205 is used as a reagent container storage part to store and keep warm a plurality of reagent containers 204 containing various reagents for sample analysis, and is covered by the reagent tray cover 206; the thermostat tray 207, the The thermostat tray 207 accommodates a plurality of reaction containers 4 for reacting the sample and the reagent; a sample dispensing mechanism 208, which dispenses the sample from the sample container 201 to a constant temperature by rotati...

Embodiment 2

[0035] Next, a second embodiment of the present invention will be described.

[0036] Figure 4 It is an example of a flowchart of a stirring operation including the stirring frequency determination process. First, the control device 216 calculates the total volume V1+V2 of the volume V1 of the first liquid and the volume V2 of the second liquid. Next, compare the preset threshold Va with the total volume V1+V2 (step 401), and if V1+V2 is greater than Va, execute steps 303 and 304 once, and then end the stirring.

[0037] On the other hand, when V1+V2 is smaller than Va, comparison with threshold value Vb (

[0038] According to this embodiment, it is possible to finely set the number of suction-discharge agitation according to the volume of...

Embodiment 3

[0040] In this example, use Figure 5A and Figure 5B This stirring method in the dilution operation of the sample will be described. Figure 5A , B are diagrams showing the flow of the sample dilution operation.

[0041] When a sample is diluted using a conventional liquid stirring method, the sample and the diluent cannot be uniformly mixed, and the diluted sample in one container may have uneven dilution ratios. In particular, in the case of repeating the dilution process multiple times to prepare a diluted sample with a high dilution factor, if the mixed solution does not reach the desired dilution factor in the first dilution process, the dilution process is repeated multiple times. There is a possibility that a large deviation may occur between the dilution ratio of the final diluted sample and the desired dilution ratio. In this example, a diluent capable of accurately preparing a sample with a dilution ratio of 1 item and a method of inhaling, discharging, and stirr...

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PUM

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Abstract

The present invention provides a liquid stirring method, even when the amount of the mixed liquid in the container is small, it can be stirred evenly without reducing the flow rate of dispensing and analysis, and avoiding the risk of scattering and foaming. The liquid stirring method of the present invention is a liquid stirring method in which the second liquid is discharged from the dispensing probe (1) provided with the dispensing needle (3) at the tip to the reaction vessel (4) containing the first liquid. After that, stirring is carried out by sucking and discharging the mixture of the first liquid and the second liquid in the container (4) by using the dispensing probe (1). In this liquid stirring method, the first liquid and the second liquid can be The total volume of the pump can be used to change the stirring times of inhalation and exhalation. When the total volume of the first liquid and the second liquid is smaller than a preset threshold value, inhalation and discharge are repeated a predetermined number of times.

Description

technical field [0001] The invention relates to a method and a device for stirring multiple liquids such as blood. Background technique [0002] In the existing dispensing devices and automatic analysis devices, when multiple liquids such as samples, reagents, and diluents ranging from several μL to hundreds of μL are injected into one container, precipitation and separation will occur due to the difference in specific gravity . Therefore, it is necessary to stir the mixed liquid in the container after dispensing. As a common method for stirring, it is known to use a dispensing probe to suck in and discharge the mixed liquid dispensed in the container, and to perform this by convection in the container. Stirring method. For example, in Patent Document 1, it is described that after the sample or reagent sucked into the dispensing needle attached to the dispensing probe is discharged into the container, the sample or reagent discharged into the container is sucked in order t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N35/02B01F3/08B01F13/00B01F15/02G01N1/38
CPCG01N35/1016G01N2035/106G01N35/10B01F23/40B01F31/651B01F33/00B01F35/714B01F35/712B01F35/7174B01F35/75465G01N35/02B01F23/45B01F2101/23G01N1/38G01N35/1009
Inventor 佐佐木俊辅山下善宽
Owner HITACHI HIGH-TECH CORP