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Method for extracting 1, 5-dicaffeoylquinic acid in globe artichoke

A technique for extracting caffeoylquinic acid and its extraction method, which is applied in the field of extracting 1,5-dicaffeoylquinic acid from artichokes, can solve the problems of high cost, complicated extraction process, and low purity, and achieve low preparation cost , simple process, high purity effect

Inactive Publication Date: 2017-03-15
CHANGDE HUIMEI AGRI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing 1,5-dicaffeoylquinic acid extraction process is very complicated, and the cost is high, and the extraction purity is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for extracting 1,5-dicaffeoylquinic acid in artichokes, comprising the steps of:

[0017] (1) Artichoke leaves are dried and crushed, and extracted with 75% methanol through a Soxhlet extraction device;

[0018] (2) Concentrate the methanol extract in vacuo to remove methanol, then add three times of water to dilute;

[0019] (3) Use petroleum ether to extract three times to remove non-polar compounds;

[0020] (4) Adjust the pH of the extracted aqueous phase to 7.0 with hydrochloric acid, and extract three times with an equal volume of ether;

[0021] (5) Acidify the extracted aqueous phase to pH=5.0, then extract three times with ether; wash once with 1 / 3 volume of distilled water to remove impurities;

[0022] (6) Evaporate ether from the liquid obtained in the above step (5), then dissolve the obtained powder in water, and then vacuum concentrate the aqueous solution to 1 / 5 of its original volume;

[0023] (7) Add an equal volume of ethanol to the concen...

Embodiment 2

[0025] A method for extracting 1,5-dicaffeoylquinic acid in artichokes, comprising the steps of:

[0026] (1) Artichoke leaves are dried and crushed, and extracted with 75% methanol through a Soxhlet extraction device;

[0027] (2) Concentrate the methanol extract in vacuo to remove methanol, then add three times of water to dilute;

[0028] (3) Use petroleum ether to extract three times to remove non-polar compounds;

[0029] (4) Adjust the pH of the extracted aqueous phase to 6.0 with hydrochloric acid, and extract three times with an equal volume of ether;

[0030] (5) Acidify the extracted aqueous phase to pH=3.0, then extract three times with ether; wash once with 1 / 3 volume of distilled water to remove impurities;

[0031] (6) Evaporate ether from the liquid obtained in the above step (5), then dissolve the obtained powder in water, and then vacuum concentrate the aqueous solution to 1 / 5 of its original volume;

[0032] (7) Add an equal volume of ethanol to the concen...

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PUM

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Abstract

The invention discloses a method for extracting 1, 5-dicaffeoylquinic acid in globe artichoke. The method includes the steps: (1) drying and crushing globe artichoke leaves, and performing extraction through a Soxhlet extraction device by the aid of 75% methyl alcohol; (2) concentrating methyl alcohol extracting solution in a vacuum manner to remove the methyl alcohol, and adding three times of water to dilute the solution; (3) performing extraction for three times by the aid of petroleum ether; (4) adjusting pH (potential of hydrogen) of an aqueous phase after extraction to 7.0-6.0 by the aid of hydrochloric acid, and performing extraction for three times by the aid of diethyl ether with the same volume; (5) acidizing the aqueous phase after extraction until the pH of the aqueous phase is 5.0-3.0, performing extraction for three times by the aid of diethyl ether, and washing the aqueous phase once by the aid of distilled water with 1 / 3 volume; (6) evaporating the diethyl ether in liquid, dissolving powder in water, and concentrating water solution in a vacuum manner until the volume is 1 / 5 that of original water solution; (7) adding ethyl alcohol with the same volume into the concentrated water solution, and crystallizing the solution at low temperature to obtain the 1, 5-dicaffeoylquinic acid. HPLC (high performance liquid chromatography) detection purity reaches 90%. The method is simple in process and low in preparation cost, and the purified 1, 5-dicaffeoylquinic acid is high in purity.

Description

technical field [0001] The invention relates to the technical field of biological extraction, in particular to a method for extracting 1,5-dicaffeoylquinic acid in artichokes. Background technique [0002] 1,5-dicaffeoylquinic acid is the raw material for the synthesis of cynarin, which has anti-oxidation and liver-protecting effects, so it is of great significance for the extraction of 1,5-dicaffeoylquinic acid. The current extraction process of 1,5-dicaffeoylquinic acid is very complicated, and the cost is high, and the extraction purity is not high. Contents of the invention [0003] The purpose of the present invention is to provide a simple, low-cost, high-purity extraction method for 1,5-dicaffeoylquinic acid in artichokes. [0004] The object of the present invention is achieved through the following technical solutions: [0005] A method for extracting 1,5-dicaffeoylquinic acid in artichokes, comprising the steps of: [0006] (1) Artichoke leaves are dried and c...

Claims

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Application Information

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IPC IPC(8): C07C67/48C07C69/732
CPCC07C67/48C07C69/732
Inventor 邓爱华肖立安刘志文
Owner CHANGDE HUIMEI AGRI TECH