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Genetic engineering bacterial strain for directionally producing compounds having anti-tuberculosis activity and anti-tumor activity and application thereof

A technology of genetically engineered strains and compounds, applied in the direction of antineoplastic drugs, microorganism-based methods, microorganisms, etc.

Active Publication Date: 2017-03-15
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some strains can simultaneously produce multiple secondary metabolites, which is an advantage for compound diversity, but for directed target drug development, this advantage becomes a disadvantage

Method used

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  • Genetic engineering bacterial strain for directionally producing compounds having anti-tuberculosis activity and anti-tumor activity and application thereof
  • Genetic engineering bacterial strain for directionally producing compounds having anti-tuberculosis activity and anti-tumor activity and application thereof
  • Genetic engineering bacterial strain for directionally producing compounds having anti-tuberculosis activity and anti-tumor activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1. Cultivation of Streptomyces atratus SCSIO ZH16 and establishment of genetic manipulation system

[0046] 1. Solid culture of S.atratus SCSIO Zh16:

[0047] 1.1 Marine Streptomyces S.atratus SCSIO Zh16 was isolated from deep-sea sediments in the South China Sea. The strain was preserved on the slope of ISP-2 medium. The composition of ISP-2 medium was: yeast extract powder 4g, glucose 4g, malt extract powder 10g, 30g coarse sea salt, 20g agar powder, 1000mL water, pH 7.2-7.4, sterilized for later use.

[0048] 1.2 Establishment of Streptomyces atratus SCSIO ZH16 genetic manipulation system

[0049] We studied the growth and sporulation of Streptomyces atratus SCSIO ZH16 on different media, and found that it contained 20nM Mg 2+ Sporulation was good on the ISP2 medium. At the same time, the sensitivity of Streptomycesatratus SCSIO ZH16 to a variety of antibacterial drugs was studied. The tested antibacterial drugs included: apramycin (Apramycin, Apm), ampicillin, ka...

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Abstract

The invention discloses a genetic engineering bacterial strain for directionally producing compounds having anti-tuberculosis activity and anti-tumor activity and an application thereof. The genetic engineering bacterial strain is a genetic engineering bacterial strain obtained by knocking out an ialL gene or ilaR gene in a genome of streptomyces atratus SCSIO ZH16, wherein the ialL gene has the nucleotide sequence shown in SEQ ID NO.1, and the ialR gene has the nucleotide sequence shown in SEQ ID NO.2. The genetic engineering bacterial strain can produce the compounds 1, 2, 3, 4 and 5 having anti-tuberculosis activity and anti-tumor activity and shows great value in development of anti-tuberculosis drugs. Therefore, the successful construction of the genetic engineering bacterial strain for directionally producing the compounds having anti-tuberculosis activity and anti-tumor activity can accelerate the process of industrialization of the compounds and promote the development of Chinese marine drugs.

Description

technical field [0001] The invention belongs to the field of microbial genetic engineering and metabolic engineering, and in particular relates to a genetic engineering bacterial strain for directional production of anti-tuberculosis activity and antitumor activity compound cyclopeptide antibiotic ilamycins and application thereof. [0002] Background technique: [0003] Tuberculosis is a serious and globally prevalent infectious disease, ranking first among the deaths caused by infectious diseases. Currently, chemotherapy remains the mainstay of treatment for adult tuberculosis. However, due to the unique pathogenic mechanism and invasion characteristics of Mycobacterium tuberculosis, drugs that can effectively deal with Mycobacterium tuberculosis are still limited and the drug use cycle is long, accompanied by very serious side effects, poor patient compliance or Drugs cannot be taken according to the course of treatment, resulting in the emergence and rapid spread of mult...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P21/04C07K7/64A61K38/12A61P35/00A61P31/06C12R1/465
CPCA61K38/00A61P31/06A61P35/00C07K7/64
Inventor 鞠建华马俊英黄洪波张春燕贾艳玺李岩
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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