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Rapid test strip for potato viruses, and preparation method and application thereof

A potato virus and detection test paper technology is applied in the field of immunochromatographic technology to detect plant viruses, and can solve the problems of difficulty in detecting dormant potatoes and high misjudgment rate

Active Publication Date: 2017-03-15
KESHAN BRANCH OF HEILONGJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method overcomes the shortcomings of the existing conventional diagnostic methods for potato virus diseases based on symptomology, such as the high misjudgment rate, the difficulty in detecting the virus in dormant potatoes by serological methods, and the phloem virus-potato leafroll virus with less virus content; The method is suitable for the early diagnosis of various virus diseases of potato seedlings and seed potatoes, and plays an important role in the quality monitoring of potato seed and the prevention of virus diseases

Method used

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  • Rapid test strip for potato viruses, and preparation method and application thereof
  • Rapid test strip for potato viruses, and preparation method and application thereof
  • Rapid test strip for potato viruses, and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0098] A kind of potato virus rapid detection test strip, such as figure 1 , figure 2 As shown, it includes sample pad 1, gold standard pad 2, NC film 3, detection line T line 4, quality control line C line 5, water absorption pad 6 and PVC bottom plate 7, and the upper part of the PVC bottom plate 7 is pasted with NC film 3. The two ends of the upper part of the NC membrane 3 are overlapped with a gold standard pad 2 and a water-absorbing pad 6 respectively, the upper part of the gold standard pad 2 is lapped with a sample pad 1, and the detection line T line 4 is set close to the sample pad 1 On the surface of the NC membrane 3, the quality control line C line 5 is arranged on the NC membrane 3 close to the water-absorbing pad 6, and the detection line T line 4 is coated with a rabbit polyclonal antibody against potato virus, and the coating is on The concentration of the potato virus rabbit polyclonal antibody solution on the detection line T line 4 is 0.5 μ L / cm, and the...

Embodiment 2

[0111] A kind of potato virus rapid detection test strip, such as figure 1 , figure 2As shown, it includes sample pad 1, gold standard pad 2, NC film 3, detection line T line 4, quality control line C line 5, water absorption pad 6 and PVC bottom plate 7, and the upper part of the PVC bottom plate 7 is pasted with NC film 3. The two ends of the upper part of the NC membrane 3 are overlapped with a gold standard pad 2 and a water-absorbing pad 6 respectively, the upper part of the gold standard pad 2 is lapped with a sample pad 1, and the detection line T line 4 is set close to the sample pad 1 On the surface of the NC membrane 3, the quality control line C line 5 is arranged on the NC membrane 3 close to the water-absorbing pad 6, and the detection line T line 4 is coated with a rabbit polyclonal antibody against potato virus, and the coating is on Potato virus rabbit polyclonal antibody solution drawing concentration on detection line T line 4 is 1 μ L / cm, and the concentra...

Embodiment 3

[0116] A kind of potato virus rapid detection test strip, such as figure 1 , figure 2 As shown, it includes sample pad 1, gold standard pad 2, NC film 3, detection line T line 4, quality control line C line 5, water absorption pad 6 and PVC bottom plate 7, and the upper part of the PVC bottom plate 7 is pasted with NC film 3. The two ends of the upper part of the NC membrane 3 are overlapped with a gold standard pad 2 and a water-absorbing pad 6 respectively, the upper part of the gold standard pad 2 is lapped with a sample pad 1, and the detection line T line 4 is set close to the sample pad 1 On the surface of the NC membrane 3, the quality control line C line 5 is arranged on the NC membrane 3 close to the water-absorbing pad 6, and the detection line T line 4 is coated with a rabbit polyclonal antibody against potato virus, and the coating is on Potato virus rabbit polyclonal antibody solution drawing concentration on detection line T line 4 is 1.5 μ L / cm, and the concen...

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Abstract

The invention discloses a rapid test strip for potato viruses, and a preparation method and application thereof. The rapid test strip for the potato virus comprises a sample pad, a colloidal-gold pad, a NC membrane, a test line (a T line), a control line (a C line), a water absorbing pad and a PVC base plate. The preparation method comprises the following four steps: preparation of the colloidal-gold pad; preparation of the NC membrane; preparation of the sample pad; and assembling of the test strip. Application and detection comprise the following concrete steps: treatment of a sample; dropwise addition of the sample; result interpretation; etc. The potato viruses comprise PVX, PVYNTN, PVYo, PLRV, PVS, PVM, PVA and a tomato black ring virus. The test strip is convenient to use, has good applicability, needs no laboratory, no special apparatus and no operation training of professionals, can realize testing in any place with potatoes, such as a field, a potato storage cellar and a customhouse, and can be used by any potato planter.

Description

technical field [0001] The invention relates to the technical field of detecting plant viruses by using immunochromatography technology, in particular to a test strip for quick detection of potato virus and its preparation method and application. Background technique [0002] Potato is an economic crop with high application value in my country. It has been listed as one of the seven major crops in my country. It is also the most important crop variety and the main pillar industry in western China. It has a good development momentum, but the current potato yield in my country is far low. In developed countries, potato viruses are the main cause of low yield and poor quality of potatoes. There are immunochromatographic tests in China's medical testing such as early pregnancy, hepatitis B, parasites, venereal disease testing and animal disease testing such as poultry disease diagnosis, livestock epidemic disease, mouse hepatitis virus, pig trichinellosis, and canine disease viru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543
CPCG01N33/543G01N33/56983
Inventor 刘卫平李志新张微张莹莹张新宇
Owner KESHAN BRANCH OF HEILONGJIANG ACADEMY OF AGRI SCI
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