Micro-fluidic chip device for simultaneously detecting women's various typical tumor markers

Abstract

The invention relates to a micro-fluidic chip device for simultaneously detecting women's various typical tumor markers and belongs to the field of analytical test. PDMS, namely polydimethylsiloxane, is used for preparation of a substrate of a micro-fluidic chip suitable for combined detection of women's eight typical tumor markers. There are advantages in price and process, but difficulties also exist. The invention aims to overcome the difficulties. Main points of the scheme are as follows: the PDMS with an original ecological surface is selected as the substrate, and an elastic clamp with a miniature ultrasonic transducer attached to the clamping arm is, by elastic clenching, positioned at the neighboring position of a sample fluid terminal of the micro-fluidic chip, so as to reduce interfacial tension and increase interfacial compatibility by means of ultrasonic wave; meanwhile, by strong ultrasonic wave absorption power of PDMS, ultrasonic intensity is rapidly decreased progressively within a short distance such that an interfacial tension difference is formed across the two ends of the chip and thereby the sample fluid flows towards the direction of the terminal along the original hydrophobous capillary channel. The device is completely used without a micropump.

Description

technical field [0001] The invention relates to a microfluidic chip device for simultaneously detecting multiple typical tumor markers of women, belonging to the field of analysis and testing. Background technique [0002] Tumor markers (tumor markers, TM) refer to a class of substances produced by tumor cells themselves or produced by the body's response to tumor cells during the occurrence and proliferation of tumors, reflecting the existence and growth of tumors, including Proteins, hormones, enzymes (isoenzymes) and oncogene products, etc. Testing the tumor markers in the patient's blood or body fluids can detect tumors early in the tumor screening, observe the curative effect of tumor treatment and judge the prognosis of patients. Currently, the tumor markers commonly used clinically are: (1) alpha-fetoprotein (AFP) is a marker for primary liver cancer, testicular cancer, ovarian cancer and other tumors; (2) carcinoembryonic antigen (CEA) is a marker for digestive syst...

AI Technical Summary

Problems solved by technology

[0008] But it's not that simple

[0009] First, this polydimethylsiloxane material, the material referred to by the acronym PDMS, is itself a strongly hydrophobic material. Microchannels are built on this material. If the microchannels are not targeted The modification operation of the surface of the channel, then, after the overall assembly is completed, that is, after the cover is covered, because the inner surface of the micro channel in the structure occupies most of the inner surface of the liquid flow channel, then the PDMS micro channel The strong hydrophobic characteristic of the inner surface of the channel is the decisive factor, which will make it very difficult for the polar liquid flow similar to the aqueous solution to pass through, and its flow resistance is so large that even ordinary micropumps are difficult to push. Of course, If the cover sheet also chooses to use the PDMS material, then the problem is basically the same, with little difference; therefore, in the prior art, it is necessary to modify and modify the inner surface of the microchannel on the PDMS material; then , is this modification operation for the inner surface of the PDMS microchannel very troublesome? That's not the problem. What constitutes a serious technical proble...

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