Dialysis device special for regenerated silk fibroin laboratory and use method of dialysis device

A technology of regenerated silk fibroin and dialysis device, which is applied in the field of biomedical material equipment and preparation, can solve the problems of limited beaker volume, waste of manpower and time, and insufficient dialysis, so as to save manpower and time, optimize quantity and length, Effects of flexible dialysis regimens

Pending Publication Date: 2017-04-26
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, this dialysis method has at least the following disadvantages: (1) the materials of daily water basins and buckets do not meet the requirements for the preparation of medical materials, which may affect the biocompatibility of materials; (2) the container capacity is not carefully calculated, often resulting in Fluctuation of liquor ratio, dialysis effect is unstable; (3) the effect of static dialysis is insufficient; (4) open container easily causes bacterial contamination and breeding; (5) manpower and time waste are caused by manual water change; (6) ) The regenerated silk fibroin solution may not be completely immersed in water, and the dialysis is insufficient; (7) The capacity of the dialysis bag cannot be well matched with the water container, which may cause waste of the dialysis bag; (8) Excessive cutting of the dialysis bag, It will cause waste of dialysis bags; (9) there is no drainage system, which is time-consuming and laborious; (10) the volume of the beaker is limited and the dialysis is not sufficient, which limits the amount of regenerated silk fibroin prepared each time
However, after reading and comparing, it is not difficult to find that the devices described in the above three patents are significantly different from the present invention in terms of structure, function, and field

Method used

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  • Dialysis device special for regenerated silk fibroin laboratory and use method of dialysis device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Such as figure 1 As shown, the special-purpose dialysis device for the regenerated silk fibroin protein laboratory of the present embodiment includes a casing 1, and the top of the casing 1 is fixed with four fixing rods 6 for fixing the dialysis bag filled with the regenerated silk fibroin solution. Body 1 is provided with water supply system, drainage system and water outlet system, and described water supply system, drainage system and water outlet system all adopt standard size and standard parts; Described water supply system and drainage system share interface 2 and water pipe 3, and interface 2 is located at On the very center of the bottom surface of the box body 1, the interface 2 is connected to the water pipe 3, and the water pipe 3 is provided with a valve 9. The water outlet system includes a water outlet 4 arranged on the side wall of the box body 1, and the water outlet 4 is connected to the water outlet pipe 5. A cover 8 is provided on the upper side of ...

Embodiment 2

[0038] The laboratory-specific dialysis device for regenerated silk fibroin in this embodiment is the same as that in Embodiment 1.

[0039] The specific steps for using the above-mentioned regenerated silk fibroin laboratory-specific dialysis device are as follows:

[0040] Step 1: Connect the water pipe 3 to the water source, open the valve 9 of the water pipe 3 and the water source valve in turn, and pour deionized water into the tank 1 until water is discharged from the water outlet 4;

[0041] Step 2: Open the cover 8, and vertically immerse 10 dialysis bags filled with regenerated silk fibroin solution with a length of 30 cm in the water, and the upper ends of the dialysis bags are connected and fixed on the fixing rod 6 with sutures, so that the dialysis bags are maintained in a vertical state. The regenerated silk fibroin solution is completely immersed in water and starts dialysis;

[0042]Step 3: After 24 hours, the dialysis time is over, the water source valve and ...

Embodiment 3

[0045] The laboratory-specific dialysis device for regenerated silk fibroin in this embodiment is the same as that in Embodiment 1.

[0046] The specific steps for using the above-mentioned regenerated silk fibroin laboratory-specific dialysis device are as follows:

[0047] Step 1: Connect the water pipe 3 to the water source, open the valve 9 of the water pipe 3 and the water source valve in turn, and pour deionized water into the tank 1 until water is discharged from the water outlet 4;

[0048] Step 2: Open the cover 8, and vertically immerse 10 dialysis bags filled with regenerated silk fibroin solution with a length of 40 cm in the water, and the upper ends of the dialysis bags are connected and fixed on the fixing bar 6 with sutures to keep the dialysis bags in a vertical state. The regenerated silk fibroin solution is completely immersed in water and starts dialysis;

[0049] Step 3: After 24 hours, the dialysis time is over, the water source valve and the valve 9 of ...

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Abstract

The invention provides a dialysis device special for a regenerated silk fibroin laboratory and a use method of the dialysis device. The dialysis device special for the regenerated silk fibroin laboratory comprises a box body, wherein at least one fixing stick for fixing a dialysis bag for filling a regenerated silk fibroin solution is fixed at the top end of the box body; the box body is provided with a water supply system, a water drainage system and a water discharging system; the water supply system and the water drainage system share a connection opening and a water pipe; the connection opening is formed in the bottom surface of the box body and is connected with the water pipe; a valve is arranged on the water pipe; the water discharging system comprises a water outlet formed in the side wall of the box body; and the water outlet is connected with a water discharging pipe. The dialysis device is simple in structure and convenient to use; and a more flexible scheme is supplied to different scales of dialysis of the regenerated silk fibroin solution, so that the number and the lengths of the dialysis bags are optimized, and waste is avoided. The dialysis device realizes continuous, dynamic and full dialysis of the regenerated silk fibroin solution, greatly saves labor and time and reduces the risk of dialysis pollution.

Description

technical field [0001] The invention relates to a laboratory-specific dialysis device for regenerated silk fibroin and a method for using the same, belonging to the technical field of biomedical material equipment and preparation. Background technique [0002] China is a big silk producing country, and the annual output of raw silk accounts for about 70-80% of the world's total output. Silk surgical sutures have been used clinically for hundreds of years, and have produced huge economic benefits and good social benefits. With the development of silk molecular biology and the advancement of advanced technology, silk fibroin has been increasingly researched and applied in the field of biomedical materials. In addition to the above-mentioned surgical sutures, there are also artificial blood vessels, artificial skin, wound dressings, tissue engineering scaffolds, and drug sustained-release carriers. The application forms are also increasingly abundant, including woven yarns, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/34
CPCC07K14/43586
Inventor 关国平余劭婷王璐王富军于成龙吴雨芬祁思浩李晓才
Owner DONGHUA UNIV
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