Stool sample preservation liquid, preparation method and application thereof

A fecal sample and preservation solution technology, applied to the fecal sample preservation solution and its preparation and application fields, can solve the problems of cell and nucleic acid damage and degradation, low sample reuse qualification rate, harsh preservation conditions, etc. Conducive to the morphological observation of cell separation and the effect of long storage time

Inactive Publication Date: 2017-04-26
SHENZHEN HUADA GENE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the composition of feces is very complex, containing a large amount of digestive residues and various nucleic acid degrading enzymes produced by bacteria such as proteases, DNases (DNases) and RNases (RNases), various PCR inhibitors (such as bile salts, bilirubin, Humic substances and pigments), making cells and nucleic acids in stool samples extremely vulnerable to damage and degradation
However, the current method for storing feces samples has a short storage time, a low pass rate for reusing samples, harsh storage conditions, and high costs.
[0005] Therefore, the current stool sample liquid and preservation methods still need to be improved.

Method used

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  • Stool sample preservation liquid, preparation method and application thereof
  • Stool sample preservation liquid, preparation method and application thereof
  • Stool sample preservation liquid, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] According to the general method described above, prepare the stool sample preservation solution according to the formula shown in the table below. details as follows:

[0115] Take deionized water (Milli-Q ultrapure water), add ionic strength maintenance agent, buffer solution and anticoagulant, adjust the pH to 8.0, then add fixative, fixative auxiliary agent and non-ionic detergent, mix Evenly sterilized, that is.

[0116] Table 1

[0117] component name Amount added Methanol 500ml acetic acid 3.5g

[0118] Disodium edetate 1g Phosphate buffer 100ml Sodium chloride 10g Tween-20 0.5ml Deionized water 399.5ml

[0119] Among them, the formula of the phosphate buffer solution is: add sodium dihydrogen phosphate (NaH 2 PO 4 ·H 2 O) 0.9g, disodium hydrogen phosphate (Na 2 HPO 4 ·7H 2 O) 24.97g.

Embodiment 2

[0121] Referring to the method described in Example 1, the stool sample preservation solution was prepared according to the formula shown in Table 2 below. Wherein, the formula of acetate buffer is: add 12.89g acetic acid (CH 3 COOH) 0.32g, sodium acetate (CH 3 COONa).

[0122] Table 2

[0123] component name Amount added ethanol 450ml acetic acid 1g Disodium edetate 3g acetate buffer 100ml potassium chloride 1g Tween-20 0.1ml Deionized water 449.9ml

Embodiment 3

[0125] Referring to the method described in Example 1, the stool sample preservation solution was prepared according to the formula shown in Table 3 below. Wherein, the formula of the PIPES buffer solution is: add 3.02 g of piperazine-1,4-diethanesulfonic acid (PIPES) into 150 ml of deionized water.

[0126] table 3

[0127] component name Amount added Ethylene glycol 550ml acetic acid 2.5g Disodium edetate 7g PIPES buffer 150ml Sodium chloride 5g NP-40 2.5ml Deionized water 297.5ml

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Abstract

The invention discloses a stool sample preservation liquid, a preparation method and application thereof. Specifically, the stool sample preservation liquid contains: 45 vol%-60 vol% of a fixing agent; 0.1 mass%-15 mass% of a fixing agent adjuvant; 0.01 mass%-1.5 mass% of an anticoagulant; 10 vol%-15 vol% of a buffer solution; 0.01 mass%-1 mass% of an ionic strength maintenance agent; 0.01 vol%-0.5 vol% of a nonionic detergent; and the balance water. The stool sample preservation liquid provided by the invention has the characteristics of easy preparation and low cost, can preserve stool samples at room temperature, also can effectively inhibit DNA degradation and cell damage of stool samples, maintain cell shape, and can preserve samples for a long time, and the preserved samples have high qualification rate during reutilization.

Description

technical field [0001] The invention relates to a stool sample preservation solution and a preparation method and application thereof. Background technique [0002] Non-invasive samples such as feces, hair, saliva, and urine are widely used in forensic science, wildlife research, and clinical research. Among them, feces samples are an effective means of clinical testing and the most commonly used in general surveys. They are simple, low-cost, non-invasive, It is suitable for the characteristics of large-scale crowd screening. With the development of molecular biology, the occurrence and development of tumors are attributed to related gene mutations, and the exfoliated cells in feces contain mutated genes that are closely related to colorectal cancer. Gene detection in feces is expected to become a tool for screening and diagnosing gastrointestinal tumors A new approach to early screening. According to reports, an American company has developed a stool DNA (Stool-basedDNA, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28G01N2001/305
Inventor 赵艳敏易吉朱家楼
Owner SHENZHEN HUADA GENE INST
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