Method for screening tobacco plants resistant to tomato spotted wilt virus

A technology of tomato spotted wilt virus and screening method, which is applied in the field of screening tobacco plants resistant to tomato spotted wilt virus, can solve the problem that it is difficult to meet the needs of tobacco resistance identification, the inoculation efficiency cannot meet the resistance identification, and reduce the initial concentration of virus inoculation To improve the efficiency of disease resistance identification, facilitate the long-term storage of living organisms, and facilitate the identification of disease resistance

Active Publication Date: 2017-05-10
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after specific operations, it was found that the efficiency of direct inoculation of tobacco leaves with tomato spotted wilt virus collected from the field was only 60-70%, which is difficult to meet the needs of tobacco resistance identification
It is speculated that the reason is that tomato spotted wilt virus will produce a necrotic phenotype on crops, and the virus concentration in the cells in the necrotic area is low, so the initial concentration of virus inoculation is reduced, and the inoculation efficiency cannot meet the needs of resistance identification

Method used

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  • Method for screening tobacco plants resistant to tomato spotted wilt virus
  • Method for screening tobacco plants resistant to tomato spotted wilt virus
  • Method for screening tobacco plants resistant to tomato spotted wilt virus

Examples

Experimental program
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Effect test

Embodiment 1

[0047] A screening method for tobacco plants resistant to tomato spotted wilt virus, comprising the steps of:

[0048] Step (1), Nicotiana benthamiana seedlings: sow the seeds of Nicotiana benthamiana on a floating seedling tray with a tobacco floating seedling raising substrate for 30-35 days, remove weak seedlings, select seedlings with uniform growth, and ensure that the seedlings are kept in the floating tray. Each hole has a vigorously growing Nicotiana benthamiana seedling;

[0049] Step (2), seedling cultivation of tobacco seedlings to be tested for disease resistance: 15 days after sowing of Nicotiana benthamiana, sow the tobacco seeds to be identified for disease resistance on a floating seedling tray with a tobacco floating seedling substrate for 30-35 days, Eliminate weak seedlings, select seedlings with uniform growth, and ensure that there is a robust disease-resistant tobacco seedling to be inspected in each hole in the floating plate;

[0050] Step (3), prepara...

Embodiment 2

[0068] A screening method for tobacco plants resistant to tomato spotted wilt virus, comprising the steps of:

[0069] Step (1), Nicotiana benthamiana seedlings: Sow the seeds of Nicotiana benthamiana on a floating seedling tray with a tobacco floating seedling raising substrate for 35-40 days, remove weak seedlings, select seedlings that grow in a uniform manner, and ensure that the seedlings are kept in the floating tray. Each hole has a vigorously growing Nicotiana benthamiana seedling;

[0070] Step (2), seedling cultivation of tobacco seedlings to be tested for disease resistance: 15 days after sowing of Nicotiana benthamiana, sow the tobacco seeds to be identified for disease resistance on a floating seedling tray with a tobacco floating seedling substrate for 35-40 days, Eliminate weak seedlings, select seedlings with uniform growth, and ensure that there is a robust disease-resistant tobacco seedling to be inspected in each hole in the floating plate;

[0071] Step (3...

Embodiment 3

[0089] A screening method for tobacco plants resistant to tomato spotted wilt virus, comprising the steps of:

[0090] Step (1), Nicotiana benthamiana seedlings: sow the seeds of Nicotiana benthamiana on a floating seedling tray with a tobacco floating seedling raising substrate for 32-38 days, remove weak seedlings, select seedlings that grow uniformly, and ensure that the seedlings are kept in the floating tray. There is a strong growing Nicotiana benthamiana seedling in each hole;

[0091] Step (2), seedling cultivation of tobacco seedlings to be tested for disease resistance: 15 days after sowing of Nicotiana benthamiana, sow the tobacco seeds to be identified for disease resistance on floating seedling trays with tobacco floating seedling substrates for 32-38 days, Eliminate weak seedlings, select seedlings with uniform growth, and ensure that there is a robust disease-resistant tobacco seedling to be inspected in each hole in the floating plate;

[0092] Step (3), prepa...

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Abstract

The invention relates to a method for screening tobacco plants resistant to tomato spotted wilt virus, belonging to the field of plant protection technology. The method includes five steps: nicotianabenthamiana seedling raising, preparation of resistance identification for tobacco seedlings, preparation of inoculation buffer, inoculation of tobacco seedlings and identification of disease resistance. The principle of the invention is that before the tobacco disease resistance identification is carried out, the field tomato spotted wilt virus source is activated on the nicotianabenthamiana, and the diseased tissue of the tobacco is collected as a poison source to carry out disease resistance identification.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a screening method for tobacco plants resistant to tomato spotted wilt virus. Background technique [0002] Tomato Spotted Wilt Virus (TSWV) has posed a huge threat to tobacco production. More than 60% of serious losses can be caused in local areas, and there is a tendency to expand and aggravate. The existing main flue-cured tobacco varieties can be infected by tomato spotted wilt virus, which becomes a potential threat to the epidemic and outbreak of tomato spotted wilt virus. Due to the characteristics of its transmission medium, thrips, which have a short development cycle, small individuals and easy to hide, and are prone to resistance to insecticides, it is difficult to achieve ideal control effects with existing control measures. Therefore, breeding of resistant TSWV's flue-cured tobacco varieties are the most economical and effective means. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G31/00
CPCA01G22/00A01G31/00
Inventor 黄昌军刘勇于海芹方敦煌肖炳光陈学军童治军
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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