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Method for rapidly identifying aphid resistance of mung beans

An identification method and mung bean technology, applied in the fields of botanical equipment and methods, horticulture, plant cultivation, etc., can solve problems affecting crop yield and quality, sooty disease, etc., to reduce the loss of yield and quality, ensure repeatability and The effect of reliability

Active Publication Date: 2017-05-10
INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And because the insect excretes a large amount of "honeydew", it causes coal pollution disease, and can spread more than 40 kinds of plant virus diseases, seriously affecting crop yield and quality

Method used

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  • Method for rapidly identifying aphid resistance of mung beans
  • Method for rapidly identifying aphid resistance of mung beans
  • Method for rapidly identifying aphid resistance of mung beans

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0030] The preparation of embodiment 1 for testing bean aphid

[0031] 1) Mass rearing of bean aphids indoors: Bean aphids are collected from mung beans in the field and subcultured on broad bean seedlings grown in our laboratory at a temperature of (23±1)°C and a photoperiod of 16L:8D.

[0032] 2) Preparation of the tested bean aphid: the tested nymphs were cultivated in an artificial light incubator by using the improved leaf disc method. Specifically: put fresh mung bean leaves in a petri dish containing an agar layer (1.5%) after soaking them in Pasteur disinfectant for 10 minutes, select healthy adult aphids and place them on the leaves, cover the lid, and seal it with a parafilm. Prevent bean aphids from crawling out. After 24 hours, the adult aphids in the petri dish were taken out. After the 4th day, 4-day-old nymphs were taken for artificial inoculation experiment. The aphid culture conditions are: the temperature is (23±1)°C, and the photoperiod is 16L:8D.

Embodiment 2

[0033] Example 2 Indoor insect resistance identification

[0034] Each mung bean variety was used as the experimental material, and when the mung bean grew to the stage of two leaves and one core, 2-3 wingless aphids were inoculated on the front leaves. After 7 days of inoculation with aphids, the number of aphids on each mung bean plant was counted using the counting method, and the average number of aphids on every 20 plants of the same variety of broad bean was obtained, and the resistance evaluation was carried out according to the following grading standards,

[0035] Grading is based on the ratio of the average amount of aphids per plant per plant, and is divided into 5 levels:

[0036] 1) Level 0: the average ratio of aphids per mung bean plant is 0-0.25;

[0037] 2) Level 1: the average ratio of aphids per plant of mung bean is 0.26-0.50;

[0038] 3) Level 2: The average ratio of aphids per plant of mung bean is 0.51-0.90;

[0039] 4) Grade 3: the average ratio of a...

Embodiment 3

[0042] Example 3 mung bean resource resistance identification result

[0043] Using the above method, the 4 mung bean varieties used in production were identified and evaluated for resistance to soybean aphid. The identification results are shown in Table 1.

[0044] Table 1 Identification results of indoor resistance of mung bean to bean aphid

[0045] Variety Average ratio of aphids per plant Degree of resistance Jilv 2 0.4208 R Zhonglv No. 3 0.5372 MR Zhonglv No. 1 0.9094 S Weilv 05-08 1.6347 HS

[0046] It can be seen from the above table that there are significant differences in the aphid resistance performance of the tested varieties, which proves that the identification method provided by the present invention can quickly and accurately distinguish the mung bean aphid-resistant varieties and the bean aphid-susceptible varieties.

[0047] It can be seen that for the identification of aphid resistance of varieties, the meth...

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Abstract

The invention discloses a method for rapidly identifying the aphid resistance of mung beans. The method comprises the following steps of: carrying out surface disinfection on mung bean seeds; dibbling the mung bean seeds subjected to the surface disinfection into a culture disc; alternately culturing under illumination and darkness; observing growth conditions of mung bean seedlings at fixed time; when the mung beans grow to a two-leaf and one-heart period, inoculating 2-3 apterous aphids on front leaves; and observing an insect susceptible condition. The growth and reproduction of the aphids are greatly influenced by seasons; environment conditions of a culture room are relatively stable and an identification system is a completely closed system, so that the repeatability and reliability of an identification result can be guaranteed.

Description

technical field [0001] The invention relates to the technical field of crop resistance breeding, in particular to a method for rapid identification of mung bean aphid resistance. Background technique [0002] Bean aphid (Aphis craccivora Koch) belongs to Hemiptera Aphididae, also known as alfalfa aphid and peanut aphid. It is widely distributed in the world, and its host plants can reach more than 200 species, mainly including cowpea, kidney bean, broad bean, pea, mung bean, soybean, peanut , alfalfa, etc., are important pests of legumes. The insect uses piercing-sucking mouthparts to suck the juice from tender stems, young shoots, top young leaves, heart leaves, flower organs and pods. When the damage is severe, the plants grow poorly and the leaves shrink, which affects flowering and fruiting. And because the insect excretes a large amount of "honeydew", it causes sooty disease, and can spread more than 40 kinds of plant virus diseases, seriously affecting crop yield and...

Claims

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Application Information

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IPC IPC(8): A01G1/00
CPCA01G22/00
Inventor 刘昌燕万正煌李阳焦春海李莉陈宏伟刘良军伍广洪王兴敏
Owner INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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