Method for quickly evaluating disease resistance of banana fusarium wilt

A technology for banana fusarium and Fusarium wilt, applied in the field of agriculture-plant protection, to achieve the effect of repeatability and reliability assurance

Inactive Publication Date: 2010-02-10
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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Other resistance identification methods with gr

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  • Method for quickly evaluating disease resistance of banana fusarium wilt

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[0016] Six varieties including Brazil, GCTCV-119, Formosana, Nongke No. 1, Guangfen No. 1 and Dongguan Dajiao were used as experimental materials. Inoculate the adventitious buds produced by sucking bud shoot tip explants of the above varieties into rooting medium: 1 / 2MS+1.0mg / l NAA+0.2mg / l IBA+30g / l sucrose+2g / l activated carbon+6g / l Agar. After 2-3 weeks of rooting culture, transfer the rooted test-tube plantlets with the leaves beginning to unfold to MIS for pre-cultivation, use a 150ml Erlenmeyer flask as a culture container, and inoculate one test-tube plantlet per bottle. After the rooting test-tube plantlets of the above varieties are grown on the MIS medium for 1-2 weeks, small plants with a plant height of 4.5-5 cm, the number of unfolded leaves on the upper part of the plant ≥2, and the number of roots ≥3 are selected to inoculate Foc. During inoculation, open the sealing film of the triangular flask on a clean workbench, and use tweezers to pre-soak the spore suspen...

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Abstract

The invention relates to a method of quickly evaluating disease resistance of banana fusarium wilt, belonging to the technical field of plant protection of agriculture. The invention has the followingsteps: transferring test-tube plantlets which grow for 2-3 weeks in a root culture medium to an improved culture medium MIS to pre-culture for 1-2 weeks; selecting plantlets with the height of 4.5-5cm, the number of blades unfolded on the upper part of plant being more than or equal to 2 and the number of roots being more than or equal to 3 to inoculate Fusarium oxysporum f. sp. cubense (Foc) ona super-clean worktable in the phase of root culture of fast in vitro propagation of banana; placing a culture vessel in a tissue culture room with the temperature of 25 plus or minus 2 DEG C to observe the condition of pathogenesis; evaluating disease grades of singleplant rooted test-tube plantlet according to the disease evaluation grade from grade 0 to grade 6 24 days after inoculating FOC. Compared with the present widely adopted pot culture systems and water culture systems, the evaluation method provided in the invention is not only able to be closely linked with the research of breeding for disease resistance of banana, but also has the characteristics of accuracy and fast speed.

Description

technical field [0001] The invention relates to a method for quickly identifying the disease resistance of Fusarium wilt of banana, especially under the condition of in vitro culture, by improving the medium formula and inoculating the pathogenic bacteria in the rooting culture stage, quickly and effectively Screen banana disease-resistant germplasm resources and breeding materials to improve the efficiency of banana fusarium wilt-resistant breeding. The invention belongs to the technical field of agriculture-plant protection. Background technique [0002] Banana fusarium wilt (also known as Panama disease, yellow leaf disease) is one of the main diseases that threaten banana production in my country and even the world in recent years. Widely regarded as one of the most devastating plant diseases of all time, the disease is caused by the soil fungus Fusarium oxysporum f. sp. cubeense (E.F. Smith) Snyd. & Hans (Foc). Four physiological races of Foc have been confirmed, amon...

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Application Information

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IPC IPC(8): C12Q1/18C12R1/77
Inventor 吴元立易干军彭新湘
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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