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A zwitterion-modified multifunctional mn 3 o 4 Nanoparticle Magnetic Resonance Imaging Contrast Agent and Its Preparation and Application

A magnetic resonance imaging and nanoparticle technology, applied in nanotechnology for sensing, nanomedicine, nanotechnology, etc., can solve problems such as no discovery, achieve simple and easy operation, improve imaging effect, and high enrichment amount Effect

Inactive Publication Date: 2020-05-29
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Retrieval of literature at home and abroad has not yet found information on the preparation of Na by one-step solvothermal synthesis. 3 Cit stabilized and L-Cysteine ​​modified Mn 3 o 4 Nanoparticles and related reports on MRI diagnosis of tumor models in vivo

Method used

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  • A zwitterion-modified multifunctional mn <sub>3</sub> o <sub>4</sub> Nanoparticle Magnetic Resonance Imaging Contrast Agent and Its Preparation and Application
  • A zwitterion-modified multifunctional mn <sub>3</sub> o <sub>4</sub> Nanoparticle Magnetic Resonance Imaging Contrast Agent and Its Preparation and Application
  • A zwitterion-modified multifunctional mn <sub>3</sub> o <sub>4</sub> Nanoparticle Magnetic Resonance Imaging Contrast Agent and Its Preparation and Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] 0.4812g Mn(acac) 3 Uniformly dispersed in 25mL DEG, stirred in air atmosphere at 70°C for 3 hours, then 0.075g Na 3 Cit was added to the above solution, and the mixed solution was allowed to continue to stir for 1 hour, then transferred to a high-pressure reactor, and reacted for 24 hours at 180 ° C; 2 L of distilled water was used for each dialysis, and the water was changed once), then the supernatant was removed, and the dialysis bag with a molecular weight cut-off of 5000 was continued for 3 days (2 L of distilled water was used for each dialysis, and the water was changed 8 times in total), and the dialysis was completed , take out part of the dialysate and vacuum freeze-dry, take an appropriate amount of Mn 3 o 4 -Cit powder is used for X-ray diffraction detection and FTIR testing. By comparing and analyzing X-ray diffraction patterns with standard crystal structure cards (such as figure 1 ), one-step solvothermally synthesized material and standard Mn 3 o 4...

Embodiment 2

[0081] In order to characterize the size and morphology of the prepared nanoparticles, take 5 μL of the Mn prepared in Example 1 of the present invention 3 o 4 -The Cit-PEG-Cys solution was dropped on the surface of the copper grid and dried in the air for TEM test (such as image 3 shown). TEM results showed that Mn 3 o 4 -Cit-PEG-Cys nanoparticles ( image 3 a and image 3 The morphology of b) is spherical or nearly spherical, and the diameter distribution is relatively uniform, and the diameter is about 2.7nm.

[0082] Take by weighing three kinds of materials of embodiment 1: Mn 3 o 4 -Cit, Mn 3 o 4 -Cit-PEG and Mn 3 o 4 -Cit-PEG-Cys 2-4mg carries out thermogravimetric analysis (such as Figure 4 shown). TGA test results showed that the modified Mn 3 o 4 -The weight loss of Cit nanoparticles is 40.91% ( Figure 4 a), press Mn 3 o 4 -Cit and Mal-PEG-NH 2 Mass ratio 5:1 modification, and Mal-PEG-NH 2 and L-Cysteine ​​molar ratio 1:2 modification, the cont...

Embodiment 3

[0088] In order to ensure that the nanoparticles prepared by the present invention can be safely used for in vivo bioimaging diagnosis, the prepared Mn 3 o 4 -Cit-PEG-Cys and control material Mn 3 o 4 - Hemocompatibility of Cit-PEG. According to the Mn measured in Example 2 3 o 4 -Cit-PEG-Cys and Mn 3 o 4 - the manganese concentration of the Cit-PEG solution, adding a certain amount of sodium chloride (NaCl) respectively to prepare physiological saline solutions of different materials (the mass concentration of NaCl is 0.9%). Next, physiological saline was added to form a mother solution with a Mn concentration of 1.6 mM. Then use normal saline to prepare physiological saline solutions with Mn concentrations of 0.1, 0.2, 0.4, 0.8 and 1.6 mM in sequence. Take an appropriate amount of human fresh blood, first centrifuge (2000rpm, 5min) to remove the supernatant, then wash the red blood cells 5 times with normal saline, collect healthy red blood cells and dilute them 10 t...

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Abstract

The invention relates to a zwitter-ion modified multifunctional Mn3O4 nanometer particle magnetic resonance imaging contrast agent, a preparation and an application thereof. Sodium citrate Na3Cit is coated on the manganous-manganic oxide Mn3O4 nanometer particle and then Mal-PEG-NH2 and L-cysteine molecules are connected with the nanometer particle surface in turn, so that the contrast agent is generated. The preparation comprises the following steps: dissolving Mal-PEG-NH2 in water, dropwise adding an activated Mn3O4-Cit solution, stirring and dialyzing, thereby acquiring a Mn3O4-Cit-PEG solution, and then dissolving the L-cysteine in water, dropwise adding into the Mn3O4-Cit-PEG solution, stirring and dialyzing, thereby acquiring the contrast agent. The method provided by the invention is simple in process, mild in reaction condition and easy in operation. The prepared Mn3O4 nanometer particle can long-term stably disperse in water and is free from agglomeration phenomenon.

Description

technical field [0001] The invention belongs to the field of contrast agent materials and their preparation and application, in particular to a zwitterion-modified multifunctional Mn 3 o 4 Nanoparticle magnetic resonance imaging contrast agent and its preparation and application. Background technique [0002] Cancer, also known as malignant tumor in medical terms, has directly or indirectly affected the lives of many people and has become the number one killer threatening human health. Therefore, early diagnosis and treatment become the key to cure cancer. In the early diagnosis of tumors, traditional imaging techniques can only understand tumor volume and anatomical location, while molecular imaging techniques can obtain more detection parameters, such as the evaluation of tumor growth kinetics, the detection of molecular abnormalities before malignant transformation, tumor cell Markers, etc., and in vivo molecular imaging can realize the study of pathogenesis without da...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/18A61K49/12A61K49/10B82Y5/00B82Y15/00
CPCA61K49/10A61K49/126A61K49/1833A61K49/186B82Y5/00B82Y15/00
Inventor 史向阳王鹏胡勇周本青徐凡丽张贵祥杨嘉
Owner DONGHUA UNIV
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