Biomarker combination and kit for predicting breast cancer and use method

A biomarker, breast cancer technology, applied in biochemical equipment and methods, microbial determination/inspection, recombinant DNA technology, etc., can solve the problems of low sensitivity and low accuracy, achieve good repeatability, fast operation, The effect of good feasibility and predicted effect

Active Publication Date: 2017-05-10
HUNAN SHENGWEI GENE TECH
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Problems solved by technology

[0005] The present invention provides a biomarker combination, kit and method for predicting breast cancer to solve the problem of low sensitivity and low accuracy of existing biomarkers when indicating the methylation state and methylation rate of DNA. question

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  • Biomarker combination and kit for predicting breast cancer and use method
  • Biomarker combination and kit for predicting breast cancer and use method
  • Biomarker combination and kit for predicting breast cancer and use method

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Embodiment Construction

[0039] Reference will now be made in detail to the exemplary embodiments, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, the same numerals in different drawings refer to the same or similar elements unless otherwise indicated. The implementations described in the following exemplary examples do not represent all implementations consistent with the present invention. Rather, they are merely examples of apparatuses and methods consistent with aspects of the invention as recited in the appended claims.

[0040] Telomere is a small piece of DNA-protein complex with a highly repetitive TTAGGG sequence at the end of eukaryotic linear chromosomes, which has the characteristics of maintaining chromosome integrity and controlling cell separation cycle. Telomeres exist in any human DNA sample that can be extracted. Every time a cell divides, the telomere length of each chromosome will gradually become ...

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Abstract

The invention provides a biomarker combination and a kit for predicting breast cancer. Primer design genes are chosen from four types of telomere genes with the most obvious methylation rate difference in breast cancer tissues and normal tissues. According to the promoter regions and coding regions of the four types of telomere genes, methylation primers and qPCR (Quantitative Polymerase Chain Reaction) primers of a target gene are respectively designed, and thereby a methylation primer combination and a qPCR primer combination are formed. Since the biomarker combination provided by the invention is designed according to the telomere genes with the most obvious methylation rate difference in the breast cancer tissues and the normal tissues, when whether a sample is breast cancer is assayed, the biomarker combination can specifically capture and amplify specific DNA fragments in the assayed sample, so that the sensitivity of assay is increased, and the biomarker combination has good noise immunity, flexibility and accuracy, and can be used in the early prediction of the breast cancer, the evaluation and assay of medicines for treatment and the detection of prognosis recurrence. The method for predicting the breast cancer provided by the invention has the advantages of quickness in operation, simplicity, convenience and good feasibility and prediction effect.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a biomarker combination, a kit and a use method for predicting breast cancer. Background technique [0002] With the development of today's society, malignant tumors have become one of the main killers of human beings. As for female malignant tumors, because breast cancer is an important cause of women's morbidity and death worldwide, breast cancer ranks first among female malignant tumors. A large number of studies have shown that the cause of breast cancer is mostly the result of the joint action of environmental factors and genetic factors, while hereditary breast cancer only accounts for 5-10% of all breast cancers, so most breast cancers cannot pass breast cancer. Mutation detection of susceptibility genes to determine whether breast cancer lesions occur. [0003] Recent studies have found that DNA (deoxyribonucleic acid, deoxyribonucleic acid) methylation sta...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6858C12Q1/6886C12Q2600/154C12Q2600/158C12Q2525/191C12Q2531/113C12Q2535/125
Inventor 王军郭鑫武彭厘旻戴立忠
Owner HUNAN SHENGWEI GENE TECH
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