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(e)-2,4-dihydroxy-6-propenylbenzaldehyde and its preparation method and use

A technology of propenylbenzaldehyde and dihydroxy, which is applied in the field of -2,4-dihydroxy-6-propenylbenzaldehyde and its preparation, can solve the problem of no inhibition of virus, no inhibition of NS3/4A protease activity, etc. problems, to achieve the effect of green environmental protection technology, short preparation cycle and convenient source of materials

Active Publication Date: 2020-01-10
SHANGHAI INST OF PHARMA IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 2,4-Dihydroxy-6-[(1'E,3'E)-1',3'-pentadienyl]benzaldehyde has been reported to promote the proliferation of CHO and HeLa cells, but it has not been found to inhibit NS3 / 4A protease activity, even no effect on virus inhibition

Method used

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  • (e)-2,4-dihydroxy-6-propenylbenzaldehyde and its preparation method and use
  • (e)-2,4-dihydroxy-6-propenylbenzaldehyde and its preparation method and use
  • (e)-2,4-dihydroxy-6-propenylbenzaldehyde and its preparation method and use

Examples

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Embodiment 1

[0040] Example 1 Preparation of compound (E)-2,4-dihydroxy-6-propenylbenzaldehyde with the structure shown in formula I

[0041]

[0042] 1. Fermentation of Aspergillus terreus PF26 strain:

[0043] The Aspergillus terreus (Aspergillus terreus) PF26 strain spores preserved in the glycerol tube (40% aqueous glycerol solution, the percentages are percent by volume) were inoculated into a 250mL Erlenmeyer shaker flask containing 50mL seed culture medium, at a temperature of 30°C and 220r / min shaking culture for 24h, as the seed solution.

[0044] Seed medium: yeast extract 5g / L, peptone 10g / L, glucose 20g / L, experimental water, pH 5.5.

[0045] Inoculate 1 mL of seed liquid into a 750 mL Erlenmeyer shaker flask (containing 200 mL of fermentation medium), and culture for 168 hours at 30°C and 220 r / min. A total of 30L fermentation broth was enriched.

[0046] Fermentation medium: glucose 30g / L, (NH 4 ) 2 SO 4 20g / L, yeast extract 10g / L, MgSO 4 ·7H 2 O 1g / L, FeSO 4 ·7...

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Abstract

The invention discloses (E)-2,4-dihydroxyl-6-allylbenzaldehyde as well as a preparation method and application thereof. The preparation method of the compound comprises the following step of fermenting and culturing aspergillus terreus PF26 to obtain a fermented solution to obtain the product. The compound has a certain inhibition effect on hepatitis c virus NS3 / 4A; and the preparation method of the compound has the advantages of convenient material source, short preparation period, greenness and environment friendliness and simple process.

Description

technical field [0001] The present invention relates to (E)-2,4-dihydroxy-6-propenyl benzaldehyde and its preparation method and use. Background technique [0002] About 150 million people in the world are infected with hepatitis C virus (Hepatitis C Virus, HCV), and there are 3 to 4 million new cases every year. After infection with HCV, some patients eventually develop liver cirrhosis or even liver cancer. It is estimated that about 500,000 people die from HCV infection and related diseases every year, so the development of anti-HCV drugs is imminent. The NS3 / 4A protease of HCV is a serine protease that can hydrolyze the connection between the HCV precursor protein NS3-NS4A-NS4B-NS5A-NS5B and determine the maturity of each functional protein of the virus. In addition, the protease can also cut the corresponding host factors For example, Toll / IL-1 receptor domain containing adapter inducing IFN-β (TRIF) and mitochondrial antiviral signaling protein (MAVS) against the natu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C47/565C07C45/79C12P7/24A61K31/11A61P31/14C12R1/66
CPCC07C45/79C07C47/565C12P7/24
Inventor 朱宝泉潘芝弟林军胡海峰周斌
Owner SHANGHAI INST OF PHARMA IND CO LTD