Bacillus and chlorella mixture, and preparation method and application of bacillus and chlorella mixture

A technology of bacillus and chlorella, which is applied in the field of a mixture of bacillus and chlorella and its preparation field, and can solve the problems of unsatisfactory reproduction rate, unfavorable growth and reproduction of chlorella, and low light transmittance of water body. , to achieve the effect of enhancing resistance, inhibiting growth and increasing dissolved oxygen content

Inactive Publication Date: 2017-05-24
HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD
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Problems solved by technology

However, its photosynthesis can only use the inorganic salt components in the water body, and has no effect on organic matter such as feces and residual bait. Especially in the late breeding period, the accumulation of organic matter in the water body and the low light transmittance of the water body are not conducive to the growth and reproduction of Chlorella. , so Chlorella used alone, its reproduction rate can not achieve the desired effect

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  • Bacillus and chlorella mixture, and preparation method and application of bacillus and chlorella mixture
  • Bacillus and chlorella mixture, and preparation method and application of bacillus and chlorella mixture
  • Bacillus and chlorella mixture, and preparation method and application of bacillus and chlorella mixture

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Embodiment Construction

[0027] The technical scheme of the present invention is described further below:

[0028] A preparation method of bacillus and chlorella mixture, the method comprises the following steps:

[0029] (1) Activating Bacillus species, using LB medium, pH 7-7.5, autoclaving at 121°C for 20 minutes, and culturing at 30°C for 18 hours;

[0030] (2) The first-level and second-level liquid expansion culture of bacillus adopts 50L fermenter to cultivate, and inoculates by 20% inoculum;

[0031] (3) Centrifuge the Bacillus bacillus secondary cultured at 4000rpm, collect the thalline, and dilute the thalline concentration to 5.0×10 with LB medium 9 CFU / mL;

[0032] (4) To activate the chlorella algae, use BG11 culture medium, pH6-8, sterilize at 121°C for 20 minutes, inoculate the algae at a ratio of 1:2, and cultivate it for 5 days at a temperature of 25°C and a light of 10000 lx;

[0033] (5) The primary and secondary expanded cultivation of Chlorella is cultivated in 5 tons of glass ...

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Abstract

The invention provides a bacillus and chlorella mixture. A volume ratio of bacillus to chlorella in the mixture is (1-9):3; the bacillus and the chlorella are compatible reasonably according to water quality of different cultivation periods; due to a synergistic effect of the bacillus and the chlorella, concentrations of ammonia nitrogen and nitrite in a water body can be reduced obviously; a dissolved oxygen content is increased; the mixture can effectively inhibit growth of enteric pathogenic bacteria of aquatic products, improve resistance and increase a survival rate when the mixture is applied to aquaculture of fishes, shrimps, crabs and the like.

Description

technical field [0001] The invention relates to the field of aquaculture nutrition, in particular to a mixture of bacillus and chlorella and its preparation method and application. Background technique [0002] High-density and intensive aquaculture in aquaculture has greatly increased production, but it has also brought many new problems. Studies have shown that the utilization rate of bait in the aquaculture process is low. In addition, a large amount of organic matter such as biological excrement and corpses accumulates at the bottom of the pond. , the types and numbers of harmful microorganisms increase, and the toxic substances produced by the decomposition of organic matter also increase. In addition, the pond is originally a closed and single ecosystem, and its self-purification and adjustment capabilities are weak. The cultured organisms have long been exposed to chemical oxygen demand (COD), biological oxygen demand (BOD), ammonia nitrogen, nitrate, and nitrite. In...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/12A23K50/80A23K10/18A23K10/30C12R1/07C12R1/89
CPCC12N1/12C12N1/20
Inventor 袁卫
Owner HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD
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