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Medium for saussurea involucrata in vitro cells

A culture medium and cell technology, which is applied in the field of culture medium for Saussurea in vitro cells, can solve the problems of poor culture effect of Saussurea involucrata cells, etc.

Inactive Publication Date: 2017-05-24
天津市博爱生物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a culture medium for Saussurea in vitro cells, so as to solve the technical problem of poor culturing effect of Saussurea in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A medium for isolated cells of Snow Lotus, which contains the components of MS medium, and also contains phytohormone 6-BA 0.5mg / L, phytohormone NAA 2mg / L, phenylalanine 15mg / L, Hydrolyzed casein 100mg / L, chitosan 10mg / L, methyl jasmonate 50μmol / L, salicylic acid 1mg / L, yeast extract 5g / L.

Embodiment 2

[0021] A culture medium for Saussurea in vitro cells, which contains the components of MS medium, and also contains phytohormone 6-BA 0.5mg / L, phytohormone NAA 2mg / L, phenylalanine 50mg / L, Hydrolyzed casein 200mg / L, chitosan 50mg / L, methyl jasmonate 100μmol / L, salicylic acid 10mg / L, yeast extract 10g / L.

Embodiment 3

[0023] A culture medium for isolated cells of Snow Lotus, which contains the components of MS medium, and also contains phytohormone 6-BA 0.5mg / L, phytohormone NAA 2mg / L, phenylalanine 32mg / L, Hydrolyzed casein 150mg / L, chitosan 25mg / L, methyl jasmonate 75μmol / L, salicylic acid 6mg / L, yeast extract 7g / L, white flower tea squeezed juice 1g / L.

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PUM

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Abstract

The invention extensively screens nutrient substances for saussurea involucrata cell growth by experimental means, and determines a formula according to the auto metabolic characteristics and actual use effect of saussurea involucrata. The formula adopts an MS medium as the basic component, and is added with a high dose of casein hydrolysate and methyl jasmonate, on the basis, the invention unexpectedly finds that adding of Chinese herbal medicines of specific variety and components is conducive to enhancing cell culture density. Based on the beneficial finding, Jasminum amplexicaule Buch, tea tree root and other Chinese herbal components originally used for human disease treatment are selected preferably by experimental means, the obtained medium unexpectedly acquires outstanding culture effect, so that the saussurea involucrata cell culture density is significantly increased, and at the same time the growth speed is faster. The medium provided by the invention acquires superior technical effects by innovative technical means, at the same time has the characteristics of low cost and easy realization, thus having good application prospect.

Description

technical field [0001] The invention relates to plant cell culture technology, and further relates to the design of nutritional conditions for plant cells, in particular to a culture medium for isolated cells of snow lotus. Background technique [0002] Plant cell culture technology is a culture process aimed at the expansion of cell mass and the accumulation of target metabolites based on isolated plant cells or protoplasts. For cell culture for the purpose of obtaining specific metabolites, the main process link is not only the expansion of cell mass, but also the induction of synthesis of target metabolites is more important for culture benefits. In the process of cultivating plant cells, culture conditions and nutritional conditions are the most important factors affecting yield. The culture conditions refer to the control of environmental conditions such as temperature, dissolved oxygen, light, and pH, while the nutritional conditions mainly refer to the culture medium ...

Claims

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Application Information

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IPC IPC(8): C12N5/04
CPCC12N5/0025
Inventor 夏雪城裘先临马丹
Owner 天津市博爱生物药业有限公司