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Culture medium used in glycyrrhiza isolated cells

A culture medium, in vitro technology, applied in cell culture medium, cell culture active agent, plant cells, etc., can solve problems such as poor licorice cell culture effect

Inactive Publication Date: 2017-03-08
天津市博爱生物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a culture medium for the isolated cells of Glycyrrhiza in order to solve the technical problem that the culture effect of Glycyrrhizae cells is not good in the prior art

Method used

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  • Culture medium used in glycyrrhiza isolated cells

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Embodiment 1

[0020] A culture medium for isolated cells of licorice, which has the components of MS medium, and also contains phytohormone 2.4-D 0.5mg / L, phytohormone 6-BA 0.5mg / L, and phytohormone NAA 0.5mg / L, methyl jasmonate 80μmol / L, salicylic acid 1mg / L, phenylalanine 20mg / L, tyrosine 5mg / L, cinnamic acid 5mg / L, sodium acetate 5mg / L, L-cysteine Acid 0.5mg / L, hydrolyzed casein 500mg / L, leucine 0.1mg / L.

Embodiment 2

[0022] A culture medium for isolated cells of licorice, which has the components of MS medium, and also contains phytohormone 2.4-D 0.5mg / L, phytohormone 6-BA 0.5mg / L, and phytohormone NAA 0.5mg / L, methyl jasmonate 150μmol / L, salicylic acid 10mg / L, phenylalanine 50mg / L, tyrosine 10mg / L, cinnamic acid 10mg / L, sodium acetate 10mg / L, L-cysteine Acid 2mg / L, hydrolyzed casein 800mg / L, leucine 5mg / L.

Embodiment 3

[0024] A culture medium for isolated cells of licorice, which has the components of MS medium, and also contains phytohormone 2.4-D 0.5mg / L, phytohormone 6-BA 0.5mg / L, and phytohormone NAA 0.5mg / L, methyl jasmonate 200μmol / L, salicylic acid 5mg / L, phenylalanine 35mg / L, tyrosine 7mg / L, cinnamic acid 8mg / L, sodium acetate 8mg / L, L-cysteine Acid 1.2mg / L, hydrolyzed casein 650mg / L, leucine 2.6mg / L.

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Abstract

The invention widely screens nutrient substances used for the growth of glycyrrhiza cells through an experimental method, and determines a formula according to a self-metabolism characteristic of the glycyrrhiza cells and an actual using effect. According to the formula, an MS culture medium is adopted as a fundamental component, casein hydrolysate, jasmonic acid methyl ester and phenylalanie with high dosages are added, on the basis above, it is accidentally discovered that the addition of traditional Chinese medicine with specific varieties and constituents is beneficial to the increase of cell culture density. Based on the favorable discovery above, the traditional Chinese medicine materials such as all-grass of Japanese climbing fern and creeping dichondra herb which are originally used for treating human diseases are preferably selected through the experimental method, remarkable cultivation effect is accidentally obtained by the culture medium, culture density of the glycyrrhiza cells is obviously increased, and growth speed of the glycyrrhiza cells is quickened at the same time. According to the culture medium used in the glycyrrhiza isolated cells, a superior technological effect is obtained through an innovative technological method; meanwhile, the cost is low, and the culture medium is easy to realize, thus the culture medium used in the glycyrrhiza isolated cells has a good application prospect.

Description

technical field [0001] The invention relates to plant cell culture technology, and further relates to the design of nutritional conditions of plant cells, in particular to a culture medium for isolated cells of licorice. Background technique [0002] Plant cell culture technology is a culture process aimed at the expansion of cell mass and the accumulation of target metabolites based on isolated plant cells or protoplasts. For cell culture for the purpose of obtaining specific metabolites, the main process link is not only the expansion of cell mass, but also the induction of synthesis of target metabolites is more important for culture benefits. In the process of cultivating plant cells, culture conditions and nutritional conditions are the most important factors affecting yield. The culture conditions refer to the control of environmental conditions such as temperature, dissolved oxygen, light, and pH, while the nutritional conditions mainly refer to the culture medium of ...

Claims

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Application Information

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IPC IPC(8): C12N5/04
CPCC12N5/0025C12N2500/30C12N2500/32C12N2500/76C12N2501/30C12N2501/90
Inventor 夏雪城裘先临马丹
Owner 天津市博爱生物药业有限公司