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Duck plague and escherichia coli dual PCR diagnostic kit

A diagnostic kit, Escherichia coli technology, applied in biochemical equipment and methods, resistance to vector-borne diseases, determination/examination of microorganisms, etc.

Pending Publication Date: 2017-05-24
GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, conventional diagnostic methods are difficult to make a diagnosis quickly and accurately, so it is necessary to establish a molecular biological method for the differential diagnosis of duck plague and colibacillosis

Method used

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  • Duck plague and escherichia coli dual PCR diagnostic kit
  • Duck plague and escherichia coli dual PCR diagnostic kit
  • Duck plague and escherichia coli dual PCR diagnostic kit

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Embodiment Construction

[0051] Embodiment of the present invention: duck plague and Escherichia coli double PCR diagnostic kit, including Taq polymerase, buffer solution 2×Buffer, ultrapure water, mixed primers and positive control; Taq polymerase concentration is 1U / mL, buffer solution 2× Buffer concentration is 0.5mol / L, mixed primer concentration is 10mmol / L, positive control concentration is duck plague genomic DNA, Escherichia coli genomic DNA each 10ng / L, negative control is ultrapure water; wherein, mixed primer includes primer DPV-F 5'-GCGTGATTCAGTGCCTAT-3', primer DP V-R 5'-GTCATCTCGGTATTGTATTGG-3', primer E.coli-F 5'-A GAGATGAGAATGTGCCTTC-3', primer E.coli-R 5'-GTTACCTT GTTACGACTTCAC-3'.

[0052] The positive control is duck plague genomic DNA and Escherichia coli genomic DNA.

[0053]

[0054]

[0055] SEQUENCE LISTING

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Abstract

The invention discloses a duck plague and escherichia coli dual PCR diagnostic kit. The two kinds of viruses including DPV and E.coli can be simultaneously detected in the same reaction system, and the detection amount of DPV and the detection amount of E.coli are 0.2 ng / mL and 0.1 ng / mL respectively. Through sensibility is lower than that of established single virus (average) PCR, the sensibility is higher than that of a traditional serology and Elisa detection method and meets the requirement of clinical detection; it is displayed by specificity verification results for the diagnostic kit that the amplification results of duck pasteurella multocida, duck salmonella, riemerella anatipestifer and healthy duck tissue DNA are negative, which indicates that the diagnostic kit is high in specificity and is capable of fast and accurately judging single or mixed infection of DPV and E.coli in clinic samples and capable of meeting the requirement of fast detecting samples in large batches and being used and popularized in China basic layer animal prevention and control centers or breeding enterprises.

Description

technical field [0001] The invention relates to the fields of poultry breeding and disease prevention and control, in particular to a dual PCR diagnostic kit for duck plague and Escherichia coli. Background technique [0002] Duck plague (Duck Plague), also known as duck viral enteritis (Duck Viral Enteritis, DVE), is an acute septic infectious disease caused by duck plague virus (Duck Plague Virus, DPV) in waterfowl such as ducks, geese and swans, Because of its high morbidity and mortality, it often causes significant economic losses to the poultry industry. E. coli (Escherichia coli, E coil) is one of the most common bacterial diseases of poultry. The diseases caused by local or systemic infection include E. coli sepsis, E. coli granuloma, air sac disease, poultry cellulitis, tumor Head syndrome, peritonitis, salpingitis, synovitis, panophthalmitis and yolk sac infection. [0003] With the continuous improvement of the scale of the breeding industry, the mixed infection...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68
CPCC12Q1/686C12Q1/689C12Q1/701C12Q2537/143Y02A50/30
Inventor 徐静峨王婧余波史开志杨莉吴位珩
Owner GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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