Rapid and simultaneous qualitative analysis of the activities of two transaminases in serum based on spectrometry

Abstract

The invention provides a spectrometry method for simultaneous rapid qualitative analysis of activities of two aminotransferases in serum and belongs to the field of biochemical detection. The method comprises the following steps: acquiring blood samples of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) with different activities; preparing corresponding serum samples; determining activities of ALT and AST in the serum samples by a biochemical method; determining spectrum of the serum samples; pretreating the spectral data by chemometrics; establishing a qualitative discrimination model for normal and abnormal activities of ALT and AST in an appropriate spectral modeling area by combining ranges of normal values of ALT and AST activities; evaluating performance of the established model; and carrying out qualitative analysis on whether activities of ALT and AST in unknown serum samples are normal or not by the use of the established model. The method of the invention is suitable for qualitative analysis of activities of ALT and AST in serum. By the method, subjects with abnormal detection index can be rapidly found out.

Description

technical field [0001] The present invention relates to a rapid and non-destructive analysis method for alanine aminotransferase (alanine aminotransferase, ALT) and aspartate aminotransferase (aspartate aminotransferase, AST) activities in serum, specifically involving the use of spectroscopic methods combined with chemometric techniques , and simultaneously qualitatively analyze the activities of ALT and AST in serum, belonging to the field of biochemical detection. Background technique [0002] Aminotransferase (aminotransferase) is a class of enzymes that catalyze the amino transfer between amino acids and keto acids, and participate in the synthesis and decomposition of amino acids. Among them, alanine aminotransferase (alanine aminotransferase, ALT) and aspartate aminotransferase (aspartate aminotransferase, AST) are important diagnostic markers. ALT mainly exists in liver cells, and its intracellular concentration is significantly higher than that in serum. When the ...

AI Technical Summary

Problems solved by technology

It is particularly worth noting that blood samples are biological samples obtained through destructive collection methods. Traditional biochemical testing methods destroy blood samples and cannot continue to use the same sample for testing other items.

In addition, the pretreatment is cumbersome and time-consuming, the need to use detection reagents, and easy to cause environmental pollution are all defects of traditional biochemical detection methods

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