Near infrared nondestructive test method for aspartate aminotransferase activity in serum
A non-destructive detection technology of aspartic acid amino group, which is applied in the field of clinical testing, can solve problems such as tedious and time-consuming, affect doctors' timely diagnosis and treatment of critical patients, and biochemical pollution, and achieve the effect of shortening the analysis time
Inactive Publication Date: 2016-01-27
CHONGQING MEDICAL UNIVERSITY
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Problems solved by technology
The incubation process and pretreatment process of this method are cumbersome and time-consuming, which affects doctors' timely diagnosis and treatment of critically ill patients; secondly, this method uses destructive detection technology, and serum samples can no longer be used for the determination of other clinical test indicators; in addition, this method needs to use Biochemical reagents, causing a certain degree of biochemical pollution to the environment
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[0026] 1. Instrument
[0027] AntarisII Fourier transform near-infrared spectrometer (Thermo Company, USA), the sampling device is a transmission analysis module, the signal acquisition software is RESULT3.0, and the data analysis and modeling software is TQAnalyst8.0. The enzyme activity assay instrument is BS-800M automatic biochemical analyzer (Shenzhen Mindray Company).
[0028] 2. Serum samples
[0029] 96 serum samples, 54 blood samples were collected from women and 42 blood samples were collected from men. The preparation method of the serum sample is to collect an appropriate amount of venous blood, place it in a blood collection tube containing a coagulant, wait for the blood to coagulate and stratify at room temperature, and take the supernatant to be the serum sample.
[0030] 3. Determination of AST activity reference value in serum samples
[0031] The AST activity of serum samples was measured by BS-800M automatic biochemical analyzer. The range of AST activi...
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The invention provides a rapid and nondestructive test method for aspartate aminotransferase (ASP) activity in human serum, and belongs to the field of a clinic examination. The method comprises the following steps: collecting a blood sample; preparing a serum sample; detecting a reference value of AST activity in the serum sample; measuring a near infrared transmittance spectroscopy of the serum sample by using a Fourier transform near infrared spectrometer; carrying out a data processing of the measured spectrums by using stoichiometry technology, and screening an optimal preprocessing method; selecting a reasonable near infrared spectroscopy modeling area; associating the processed spectrum data and the reference value of AST activity, establishing a quantitative predication model of AST activity in serum, and carrying out a verification of the established model; collecting and processing the near infrared transmittance spectroscopy of a serum sample to be measured according to the same method, and predicating AST activity in the serum to be measured by the established model. The method has the advantages of rapid and nondestructive test, simple operation and high accuracy.
Description
technical field [0001] The invention relates to a rapid and non-destructive analysis method for aspartate aminotransferase (aspartateaminotransferase, AST) activity in human serum, specifically relates to the rapid quantitative analysis of AST activity in serum by using near-infrared spectroscopy combined with chemometrics technology, which belongs to clinical Inspection field. Background technique [0002] Aspartate aminotransferase (aspartateaminotransferase, AST) is an intracellular functional enzyme, which is widely distributed in the heart, liver, skeletal muscle and kidney tissue cells of the human body, among which the heart muscle and liver have the highest content. AST can catalyze the transfer of an amino group from a specific amino acid (L-glutamic acid or L-aspartic acid) to the corresponding ketoacids (α-ketoglutarate and oxaloacetate). Under normal circumstances, the extracellular AST content is extremely low, and the serum content is generally only one thousa...
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IPC IPC(8): G01N21/3577G01N21/359
Inventor 范琦钟潇骁薛建江吴阮琦王以武
Owner CHONGQING MEDICAL UNIVERSITY