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Thyroglobulin enzyme linked immunosorbent diagnostic kit

A technology of thyroglobulin and globulinase, which is applied in the field of medicine and biology, can solve the problems of short storage period and poor stability, and achieve the effects of improved storage period, high accuracy, and favorable promotion and application

Inactive Publication Date: 2017-05-24
GUANGZHHOU HUAHONG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the common problems of short storage period and poor stability in existing thyroglobulin ELISA kits, the present invention provides a thyroglobulin ELISA kit, which has good stability and can be used in The validity period is 1 year at room temperature and up to 3 years at 4°C, which significantly improves the shelf life of the kit

Method used

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  • Thyroglobulin enzyme linked immunosorbent diagnostic kit

Examples

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Effect test

Embodiment 1

[0023] Embodiment 1, thyroglobulin ELISA diagnostic kit of the present invention

[0024] The kit of this embodiment consists of a thyroglobulin polyclonal antibody-coated plate, a control buffer, an enzyme labeling solution, a washing solution, a chromogenic solution, a substrate solution, a thyroglobulin standard and a stop solution;

[0025] Wherein, the preparation process of the thyroglobulin polyclonal antibody-coated plate is as follows: after diluting the thyroglobulin polyclonal antibody for coating with 0.05mol / L carbonate buffer solution of pH 9.0, press 0.1mL Coat each well into a microwell plate, and after absorbing at 4°C for 24 hours, wash the plate with 0.05mol / L pH9.0 phosphate buffer, then block with blocking solution for 18 hours at 4°C, and discard the excess block solution, and the thyroglobulin polyclonal antibody-coated plate was prepared after vacuum drying;

[0026] Described enzyme marking solution is the 15mmol / L of the chitosan containing the thyro...

Embodiment 2

[0034] Embodiment 2, thyroglobulin ELISA diagnostic kit of the present invention

[0035] The kit of this embodiment consists of a thyroglobulin polyclonal antibody-coated plate, a control buffer, an enzyme labeling solution, a washing solution, a chromogenic solution, a substrate solution, a thyroglobulin standard and a stop solution;

[0036]Wherein, the preparation process of the thyroglobulin polyclonal antibody-coated plate is as follows: after diluting the thyroglobulin polyclonal antibody for coating with 0.05mol / L carbonate buffer solution of pH 9.0, press 0.1mL Coat each well into a microwell plate, and after absorbing at 8°C for 26 hours, wash the plate with 0.05mol / L pH9.0 phosphate buffer, then block with blocking solution for 20 hours at 8°C, and discard the excess blocking solution, and the thyroglobulin polyclonal antibody-coated plate was prepared after vacuum drying;

[0037] Described enzyme labeling liquid is the 15mmol / L of the chitosan containing the thyr...

Embodiment 3

[0045] Embodiment 3, thyroglobulin ELISA diagnostic kit of the present invention

[0046] The kit of this embodiment consists of a thyroglobulin polyclonal antibody-coated plate, a control buffer, an enzyme labeling solution, a washing solution, a chromogenic solution, a substrate solution, a thyroglobulin standard and a stop solution;

[0047] Wherein, the preparation process of the thyroglobulin polyclonal antibody-coated plate is as follows: after diluting the thyroglobulin polyclonal antibody for coating with 0.05mol / L carbonate buffer solution of pH 9.0, press 0.1mL Coat each well into a microwell plate, after absorbing at 6°C for 24 hours, wash the plate with 0.05mol / L pH9.0 phosphate buffer, then block with blocking solution for 18 hours at 6°C, discard the excess blocking solution, and the thyroglobulin polyclonal antibody-coated plate was prepared after vacuum drying;

[0048] Described enzyme marking solution is the 15mmol / L of the chitosan containing the thyroglobu...

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Abstract

The invention belongs to the technical field of medical biology, and particularly relates to a thyroglobulin enzyme linked immunosorbent diagnostic kit, which is formed by a thyroglobulin polyclonal antibody-coated plate, a contrast buffer solution, an enzyme labeling solution, washing liquid, a developing solution, a substrate solution, a thyroglobulin standard substance and a stop solution. The thyroglobulin enzyme linked immunosorbent diagnostic kit has high sensitivity, high specificity, high accuracy, and favorable stability, the expiry date of the kit at the room temperature is 1 year, the expiry date of the kit at the temperature of 4 DEG C is 3 years, and the storage life of the kit is remarkably prolonged.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to a thyroglobulin ELISA diagnostic kit. Background technique [0002] Thyroid disease is a common and frequently-occurring disease in my country. Thyroglobulin (TG) is a unique antigen of the thyroid gland, which has important clinical significance, and abnormalities of TG will appear in varying degrees in the serum of patients with various thyroid diseases. Clinically, by measuring the content of TG, it is helpful for the diagnosis of thyroid disease. [0003] TG is a 660ku glycoprotein secreted by the thyroid follicular epithelium. Each TG contains about 2 thyroxine (T4) and 0.5 triiodothyronine (T3) molecules, which are stored in the follicular lumen. Lysosomes hydrolyze T4 and T3 on the surface of TG and release them into the blood, and a small amount of TG is also released into the blood, and part of TG is secreted into the blood through the thyroid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6854G01N33/6893G01N2800/046
Inventor 陈立国邹伟权张亚丽李庆祥母润红王涛苏焱
Owner GUANGZHHOU HUAHONG BIOLOGICAL TECH
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