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A kind of in-vitro culture method of Primula japonica

A technology of primrose, in vitro culture, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., to achieve the effects of tidy and consistent reproduction of offspring, large reproduction coefficient, and maintaining excellent characters

Active Publication Date: 2019-04-19
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] As a critically endangered species, the current research on Primula fragrans is still in a blank stage, and the situation is grim

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] On March 14, 2016, the young leaves of Primula japonica were taken, soaked in washing powder for 40 minutes, rinsed under running water for 30 minutes, and gently scrubbed the leaves with a soft brush. Then transfer to the ultra-clean workbench for sterilization. First use a cotton ball dipped in 75% alcohol to wipe the surface of the leaves, rinse twice with sterile water, soak the leaves in 75% alcohol for 10 seconds, rinse with sterile water three times, then treat with 0.1% mercury liter for 10 minutes, rinse with sterile water Rinse 3 times, and shake gently to make the leaves fully contact with mercury chloride. Insert MS medium, after 14 days, cut the obtained sterile leaves into 1cm×1cm size, and cut off a small amount of the edge of the leaves, insert MS+TDZ1.0mg / L+NAA0.01mg / L+carrageenan 6.0g / L+pH6.4 medium. After 18 days of cultivation, adventitious buds were induced from the wounds around the leaves, and the adventitious buds grew well and had a large num...

Embodiment 2

[0026] On March 18, 2016, the young leaves of Primula japonica were taken, soaked in washing powder for 40 minutes, rinsed under running water for 30 minutes, and gently brushed the leaves with a soft brush. Then transfer to the ultra-clean workbench for sterilization. First use a cotton ball dipped in 75% alcohol to wipe the surface of the leaves, rinse twice with sterile water, soak the leaves in 75% alcohol for 10 seconds, rinse with sterile water three times, then treat with 0.1% mercury liter for 10 minutes, rinse with sterile water Rinse 3 times, and shake gently to make the leaves fully contact with mercury chloride. Insert MS medium, after 14 days, cut the obtained sterile leaves into 1cm×1cm size, and cut off a small amount of the edge of the leaves, insert MS+TDZ1.5mg / L+NAA0.05mg / L+carrageenan 6.0g / L+pH6.4 medium. After 18 days of cultivation, adventitious buds were induced from the wounds around the leaves, and the adventitious buds grew well and had a large numb...

Embodiment 3

[0028] On March 22, 2016, the young leaves of Primula japonica were taken, soaked in washing powder for 40 minutes, rinsed under running water for 30 minutes, and gently scrubbed the leaves with a soft brush. Then transfer to the ultra-clean workbench for sterilization. First use a cotton ball dipped in 75% alcohol to wipe the surface of the leaves, rinse twice with sterile water, soak the leaves in 75% alcohol for 10 seconds, rinse with sterile water three times, then treat with 0.1% mercury liter for 10 minutes, rinse with sterile water Rinse 3 times, and shake gently to make the leaves fully contact with mercury chloride. Insert MS medium, after 14 days, cut the obtained sterile leaves into a size of 1cm×1cm, and cut off a small amount of the edge of the leaves, insert MS+TDZ1.5mg / L+NAA0.01mg / L+carrageenan 6.0g / L+pH6.4 medium. After 15 days of cultivation, adventitious buds were induced from the wounds around the leaves, and the adventitious buds grew well and had a larg...

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PUM

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Abstract

The invention discloses an in-vitro culture method for primulina glandaceistriata. The in-vitro culture method for the primulina glandaceistriata comprises the steps: step 1, taking tender leaves of the primulina glandaceistriata in springs, washing and sterilizing the tender leaves of the primulina glandaceistriata, grafting the treated leaves into an initial medium to be cultured for 14 d; step 2, slicing the sterile leaves cultured in the step 1 and grafting the sterile leaves into an adventitious bud induction culture medium to be cultured for 15-18 d; step 3, grafting adventitious buds obtained in the step 2 into a subculture medium to be cultured for 25-30 d; step 4, grafting culturing young plants obtained in the step 3 into a rooting medium to be cultured for 18-20 d; and step 5, exercising rooted seedlings obtained in the step 4 for 4-5 d, planting the exercised rooted seedlings in a substrate and culturing the planted rooted seedlings for 7-10 d. The in-vitro culture method for the primulina glandaceistriata, disclosed by the invention, has the advantages of simple operation, reasonable design, high reproductive speed, large reproduction coefficient and regular offspring and can keep the fine properties of the original variety and is not limited by seasons.

Description

technical field [0001] The invention relates to a method for in vitro cultivation of Primula japonica. Background technique [0002] Primulina glandaceistriata (Gesneriaceae) is a herbaceous perennial plant of the family Gesneriaceae. This species was published on "Phytotaxa" in 2014 and is mainly distributed in Lingchuan County, Guangxi. This species is morphologically close to Primulina dryas and Primulina beiliuensis, but its leaves have brown stripes and are distinct from these two species. This species is currently a critically endangered species. [0003] As a critically endangered species, the current research on Primula fragrans is still in a blank stage, facing a severe situation. Need a kind of reproduction speed fast at present, reproduction coefficient is big, and the reproduction offspring is neat and consistent, can keep the good character of original variety, the in vitro culture method of the Primula japonica that is not restricted by the season. Content...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 林茂王华新李进华廖美兰孙开道陈尔孙丽娜
Owner GUANGXI FORESTRY RES INST