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A kind of rapid propagation method of Caulis japonica in vitro

A fast technology, applied in the field of plant tissue culture, can solve the problems of scarcity of wild sea vine resources and short supply, and achieve the effects of reducing the rate of disinfection and pollution, improving quality, and protecting the ecological environment.

Active Publication Date: 2019-02-12
三明市农业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is a rapid in vitro propagation method of Caulis chinensis with high reproduction rate, good quality of test-tube seedlings, and large-scale breeding of Cassia spp. seedlings. The application of this method can meet the large-scale planting requirements of artificial cultivation of Cassia spp. , to solve the current situation that the wild sea wind vine resources are becoming increasingly scarce and the market is in short supply

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for rapid propagation of sea breeze in vitro, the specific steps are as follows:

[0025] ①Pretreatment, selection and disinfection of explants

[0026] Choose healthy and vigorous sea breeze vines, spray 800 times solution of 30% mancozeb suspension, spray once a week for 3 times; then cut the first 5 canes of the sea breeze vines, and then Cut the stems into 3~5cm budded stems, clean them with a detergent solution, and dry them for later use; soak them in 72% ethanol solution for 30 seconds on an ultra-clean workbench, and then rinse them with sterile water twice. The filter paper is soaked dry; then sterilized with 0.1% mercury liter solution, the first 2 sections of tender stems are disinfected for 7 minutes, and the last 3 sections of cane sections are disinfected for 10 minutes, and then rinsed with sterile water for 3 times, and then blotted dry with sterile filter paper for use;

[0027] ②Induction and cultivation of axillary buds

[0028] Transfer the explant...

Embodiment 2

[0041] A method for rapid propagation of sea breeze in vitro, the specific steps are as follows:

[0042] ①Pretreatment, selection and disinfection of explants

[0043] Choose healthy and vigorous sea breeze vines, spray 800 times solution of 30% mancozeb suspension, spray once a week for 3 times; then cut the first 5 canes of the sea breeze vines, and then Cut the stems into 3~5cm budded stems, wash them with a detergent solution, dry them for later use; soak them in 72% ethanol solution for 35 seconds on an ultra-clean workbench, and then rinse them with sterile water 3 times. The filter paper is soaked dry; then sterilized with 0.1% mercury solution, the first 2 sections of tender stems are sterilized for 9 minutes, and the last 3 sections of cane stems are sterilized for 12 minutes, and then rinsed with sterile water 4 times, and then use sterile filter paper to absorb dry for use;

[0044] ②Induction and cultivation of axillary buds

[0045] Transfer the explants treated in step...

Embodiment 3

[0058] A method for rapid propagation of sea breeze in vitro, the specific steps are as follows:

[0059] ①Pretreatment, selection and disinfection of explants

[0060] Choose healthy and vigorous sea breeze vines, spray 800 times solution of 30% mancozeb suspension, spray once a week for 3 times; then cut the first 5 canes of the sea breeze vines, and then Cut the stems into 3~5cm budded stems, wash them with a detergent solution, and dry them for later use; soak them in 72% ethanol solution for 32 seconds on an ultra-clean workbench, and then rinse them with sterile water twice. The filter paper is soaked dry; then sterilized with 0.1% mercury solution, the first 2 sections of tender stems are disinfected for 8 minutes, and the last 3 sections of cane sections are disinfected for 108 minutes, and then rinsed with sterile water for 3 times, and then dried with sterile filter paper for use;

[0061] ②Induction and cultivation of axillary buds

[0062] Transfer the explants treated in...

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PUM

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Abstract

The invention discloses an in-vitro rapid kadsura pepper stem propagation method and relates to the technical field of plant tissue culture. The method specifically comprises the following steps: (1) pretreating, selecting and disinfecting an explant; (2) performing axillary bud induction culture on kadsura pepper stem; (3) performing multiplication culture on adventitious buds of kadsura pepper stem; (4) performing multiplication strong seedling culture on the adventitious buds of kadsura pepper stem; and (5) performing rooting culture on the adventitious buds of kadsura pepper stem. According to the high-efficiency in-vitro rapid kadsura pepper stem propagation method disclosed by the invention, the multiplication rate is high, the test-tube plantlet quality is high, the large-scale production requirements of artificial cultivation of kadsura pepper stem can be met, and the current situations that the wild kadsura pepper stem resources are increasingly scarce and the demand exceeds supply in the market are avoided.

Description

Technical field [0001] The invention belongs to the technical field of plant tissue culture, and specifically relates to a method for rapid propagation of the sea breeze in vitro. Background technique [0002] The sea breeze rattan is a commonly used Chinese medicinal material in my country, and it is also an important ingredient in the secret recipe of traditional medicated diet for Shaxian snacks. According to statistics, Shaxian snack bars throughout the country consume more than 100 tons of dried sea breeze rattan products each year. However, with the rapid development of the snack industry, the demand for sea breeze rattan is increasing, and its price is also rising. After frequent mining, wild sea breeze vine resources are becoming scarce and far from meeting market needs. Artificial cultivation is the main way to solve its problems, and large-scale high-quality seedlings have become the bottleneck of artificial cultivation. Therefore, the establishment and improvement of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 周建金莫智龙雷伏贵叶炜江金兰曹奕鸯
Owner 三明市农业科学研究院