Application of tea saponin in prevention and control of aurelia
A technology of tea saponin and Haiyue, which is applied in the field of biological control, can solve problems such as blockage of the cooling system of power plants, affecting the normal operation of coastal power plants, affecting power supply, etc., and achieves a strong effect of killing toxicity
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Embodiment 1
[0026] Embodiment 1: The determination of the killing activity of tea saponin on the sculpture of the sea moon jellyfish
[0027] The jellyfish larvae used in the present invention come from the Yantai Coastal Zone Research Institute of the Chinese Academy of Sciences, and the scalloped larvae of the jellyfish are obtained through a temperature-inducing method. Set 0mg / L, 0.1mg / L, 0.5mg / L, 1mg / L, 2mg / L, 3mg / L, 4mg / L, 5mg / L, 10mg / L, 50mg / L, a total of 10 tea saponin concentration treatments group, the test solution was prepared with 0.22um filtered seawater. During the experiment, put the sputum larvae of jellyfish into a 24-well deep well plate, add 8 sauculus larvae of jellyfish for each treatment, and add 10ml of tea saponin solution to each hole. Three replicates were set up for each treatment, cultured in an incubator at 16°C, and the number of dead larvae of S. jellyfish was observed and recorded at 24h and 48h. The median lethal concentration of tea saponin on the scle...
Embodiment 2
[0029] Embodiment 2: Determination of the killing activity of tea saponin on jellyfish polyps
[0030] The jellyfish polyps used in the present invention come from Yantai Institute of Coastal Zone, Chinese Academy of Sciences. Set 0mg / L, 0.1mg / L, 0.5mg / L, 1mg / L, 2mg / L, 3mg / L, 4mg / L, 5mg / L, 10mg / L, 50mg / L, a total of 10 tea saponin concentration treatments group, the test solution was prepared with 0.22um filtered seawater. During the experiment, the larvae of the jellyfish were put into a 24-well deep-well plate, and 8 larvae of the jellyfish were added to each treatment, and 10 ml of the tea saponin solution for testing was added to each well. Three replicates were set up for each treatment, cultured in an incubator at 16°C, and the number of dead larvae of jellyfish polyp was observed and recorded at 24h and 48h. The median lethal concentration of tea saponin on polyp larvae was calculated using the probability unit method (Probit).
[0031] The results showed that, at 48...
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Abstract
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