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Separation and preparation method of lepidoptera epoxy olefin isomeride

A technology for lepidopteran epoxy olefins and isomers, which is applied in the field of separation and preparation of lepidopteran epoxy olefin isomers, can solve problems such as inhibitory pheromone activity, and achieve broad application prospects, Large preparation volume and environmentally friendly effect

Inactive Publication Date: 2017-05-31
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the research on the application of sex pheromones, it was found that the epoxy olefin isomers of some Lepidoptera insects have the phenomenon of inhibiting the activity of sex pheromones. Therefore, it is urgent to develop a method that can effectively separate and prepare epoxy olefin isomers. body method

Method used

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  • Separation and preparation method of lepidoptera epoxy olefin isomeride
  • Separation and preparation method of lepidoptera epoxy olefin isomeride
  • Separation and preparation method of lepidoptera epoxy olefin isomeride

Examples

Experimental program
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Embodiment 1

[0020] A method for separating and preparing lepidopteran epoxy olefin isomers, characterized in that it comprises the following steps:

[0021] 1) Preparation of semi-preparative liquid chromatography: connect the four-source gradient module, autosampler, detector and fraction collector in sequence, install the ultrapure silica gel normal phase preparative column, and the ultrapure silica gel normal phase preparative column parameters For: particle size particle size 5 microns, chromatographic column diameter 10 mm, chromatographic column column length 50 mm;

[0022] 2) Preparation of mobile phase: Measure 50 parts by volume of n-hexane and 0.25 parts by volume of tetrahydrofuran, add tetrahydrofuran into n-hexane, mix, and place in an ultrasonic device for 5 minutes of sonication;

[0023] 3) Connect the flow link prepared in step 2) to the semi-preparative liquid chromatography, set the detection wavelength of the semi-preparative liquid chromatography detector to 190 nm, ...

Embodiment 2

[0026] A method for separating and preparing lepidopteran epoxy olefin isomers, characterized in that it comprises the following steps:

[0027] 1) Preparation of semi-preparative liquid chromatography: connect the four-source gradient module, autosampler, detector and fraction collector in sequence, install the ultrapure silica gel normal phase preparative column, and the ultrapure silica gel normal phase preparative column parameters For: particle size particle size 5 microns, chromatographic column diameter 19 mm, chromatographic column column length 75 mm;

[0028] 2) Preparation of the mobile phase: measure 60 parts by volume of n-hexane and 5 parts by volume of tetrahydrofuran, add tetrahydrofuran into n-hexane, mix, and place in an ultrasonicator for 5 minutes;

[0029] 3) Connect the flow link prepared in step 2) to the semi-preparative liquid chromatography, set the detection wavelength of the semi-preparative liquid chromatography detector to 200 nm, and the flow rat...

Embodiment 3

[0032] A method for separating and preparing lepidopteran epoxy olefin isomers, characterized in that it comprises the following steps:

[0033] 1) Preparation of semi-preparative liquid chromatography: connect the four-source gradient module, autosampler, detector and fraction collector in sequence, install the ultrapure silica gel normal phase preparative column, and the ultrapure silica gel normal phase preparative column parameters For: particle size particle size 5 microns, chromatographic column diameter 30 mm, chromatographic column length 100 mm;

[0034] 2) Preparation of mobile phase: measure 75 parts by volume of n-hexane and 10 parts by volume of tetrahydrofuran, add tetrahydrofuran into n-hexane, mix, and place in an ultrasonic device for 5 minutes of sonication;

[0035] 3) Connect the flow link prepared in step 2) to the semi-preparative liquid chromatography, set the detection wavelength of the semi-preparative liquid chromatography detector to 215 nm, and the ...

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Abstract

The invention belongs to the technical field of semi-preparative HPLC separation and purification, and particularly relates to a separation and preparation method of a lepidoptera epoxy olefin isomeride. The method comprises the following steps: (1) preparation of semi-preparative liquid chromatography; (2) mixing of a flowing phase; (3) setting of parameters of the flowing phase; (4) separation and preparation of a sample. According to the method, an artificially synthesized C17-C23 epoxy olefin isomeride mixture can be effectively separated; the method has the characteristics of large preparation amount and high purity.

Description

technical field [0001] The invention belongs to the technical field of semi-preparative HPLC separation and purification, in particular to a method for the separation and preparation of epoxy olefin isomers of Lepidoptera. Background technique [0002] Insect sex pheromones, also known as sex pheromones, are secreted by the special secretory organs of individuals of the same sex of the same species of insects, which can be accepted by the receptors of individuals of the same sex and cause certain behavioral responses or Trace chemical substances for physiological effects (such as mate-seeking, directional courtship, mating, etc.). As an important discovery of chemical ecology in the 1960s, insect sex pheromones have been affirmed by scientists from all over the world because of their high efficiency, environmental protection, and strong specificity, and are considered "biologically rational pesticides." More than 600 sex pheromones reported and identified in Lepidoptera ins...

Claims

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Application Information

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IPC IPC(8): B01D15/26C07D301/32C07D303/04
CPCB01D15/26C07D301/32C07D303/04
Inventor 罗宗秀张新忠边磊李兆群蔡晓明陈宗懋
Owner TEA RES INST CHINESE ACAD OF AGRI SCI