Long-term preservation method of strain of botrytis cinerea

Abstract

The invention discloses a long-term preservation method of a strain of botrytis cinerea. According to the long-term preservation method, by utilizing the survival characteristics of botrytis cinerea conidia which can maintain vitality in water for a long time, the botrytis cinerea conidia are used as the storage bacteria of the strain for the long-term preservation of a strain of botrytis cinerea. The long-term preservation method comprises the steps: 1, preparation and culture of conidia; 2, purification of conidia; 3, preservation of botrytis cinerea; 4, resuscitation of botrytis cinerea. The long-term preservation method disclosed by the invention has the advantages that 1) the survival time of the strain is more than 2 years, the preservation time is long, the method does not need the annual strain management work of the roll tube culture, and also reduces the probability of strain mutation; 2) a sealed tube can be filled with the strain, so that the probability of pollution during storage is small, and the volume of the tube is small, the preservation of the strain is less, which is easy to manage.

Description

technical field [0001] The present invention relates to phytopathological techniques. Specifically, it is a long-term preservation method for Botrytis cinerea strains. Background technique [0002] Botrytis cinerea is an important disease of grapes, tomatoes, cucumbers and other crops. When the disease is severe, it can cause significant economic losses to production. These diseases are all caused by the infection of Botrytis cinerea. The scientific and effective control of botrytis cinerea depends on the basic research of this type of disease, and the effective preservation of the pathogenic bacteria of this type of disease is the basic work of many basic researches. In general laboratories, the preservation of Botrytis cinerea strains is often preserved in the form of mycelium grown on a test tube slant medium. Although this preservation method is simple and effective, it also has some disadvantages, mainly because the preservation time is relatively short; to achieve T...

AI Technical Summary

Problems solved by technology

In general laboratories, the preservation of Botrytis cinerea strains is often preserved in the form of mycelium grown on a test tube slant medium. Although this preservation method is simple and effective, it also has some disadvantages, mainly because the preservation time is relatively short; to achieve The purpose of long-term preservation of strains requires 1-2 times of tube transfer and nursing care per year. When the number of strains is large, the nursing workload is heavy, time-consuming and laborious; in addition, repeated tube transfers will increase the contamination and variation of strains probability