Method for separating and activating human peripheral blood T cells
A technology of human peripheral blood and peripheral blood, which is applied in the direction of blood/immune system cells, animal cells, vertebrate cells, etc., to achieve the effect of simple and easy method, guaranteed treatment effect and good repeatability
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[0020] The technical solution of the present invention will be further described below, but it is not limited thereto. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the protection scope of the present invention middle.
[0021] The method for the separation and activation of human peripheral blood T cells of the present invention comprises the following steps:
[0022] (1) Peripheral blood was diluted 5X with pH 7.2 phosphate buffer;
[0023] (2) Add 15 mL of peripheral blood mononuclear cell separation medium Ficoll-Paque into a 50 mL centrifuge tube;
[0024] (3) Slowly add the diluted peripheral blood to the Ficoll-Paque layer by layer, and centrifuge at 400g for 30min at room temperature;
[0025] (4) Carefully suck off the upper layer of plasma, taking care not to stir the middle mononuclear cell layer;
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