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A kind of preparation method of cytosine and guanine
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A technology of guanine and cytosine, applied in the field of biosynthesis, can solve problems such as unfavorable and safe production, and achieve the effects of low cost, low environmental pressure, and simple substrates
Active Publication Date: 2020-04-21
CHANGSHU JINDA TECH CO LTD
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[0004] Although the method of chemical synthesis takes a short time, it is not conducive to the expansion of safe production, and it also creates a certain pressure on the environment
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preparation example Construction
[0019] A method for preparing cytosine and guanine, comprising the steps of: respectively adding cytidine and guanosine to a culture medium, inserting Ergotaceae strains, first cultivating on a shaking table, and then performing static culture at room temperature to obtain Cytosine and Guanine.
[0020] The substratum described in the present invention is preferably but not limited to potato substratum or rice substratum, and multiple substratum such as wheat substratum, corn substratum, PDA substratum can also be selected, after screening, potato substratum and rice substratum transform The rate is higher, and the following will only examine both the potato medium and the rice medium.
[0021] A preferred example of a potato medium:
[0022] Take 220g of potato boiled juice and filter, dilute to 1L with water, add 25g of glucose, 4g of peptone, 1g of potassium dihydrogen phosphate, 1g of magnesium sulfate, 2g of sodium chloride, and 15mg of vitamin B1 to prepare a potato cul...
Embodiment 1
[0028] Add cytidine and guanosine 0.5g / L respectively to the potato medium, inoculate 1% of Cordyceps militaris, culture on a shaker for 3 days (150rpm, 27°C), culture at room temperature for 10 days, and detect by liquid phase. Cytosine (430mg) and Guanine (440mg), yields are 86% and 88% respectively, purity 98%
Embodiment 2
[0030] Add 3 g / L of cytidine and guanosine to the potato medium, insert 15% of Cordyceps genus Cordyceps, culture on a shaker for 4 days (150 rpm, 27°C), culture at room temperature for 30 days, and perform liquid phase detection. The yields of cytosine (2.58g) and guanine (2.61g) were 86% and 87%, respectively, and the purity was 98%.
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Abstract
The invention belongs to the field of biosynthesis, and particularly discloses a method for preparing cytosine and guanine. The method comprises the following steps: respectively adding cytidine and vernine into a culture medium, and inoculating a clavicipitaceae strain; performing shake cultivation and room-temperature standing culture to prepare the cytosine and guanine. According to the preparation method, enzymatic conversion substrates cytidine and vernine generated in a microorganism growing process are utilized to generate the cytosine and guanine, the conversion rate is 85 percent, and the purity is over 98 percent. The method does not need other chemicals or reagent and does not have solid-liquid-gas waste generation, and the whole process has static culture and low energy consumption. The method is suitable for large-scale expandable production.
Description
technical field [0001] The invention relates to a preparation method of cytosine and guanine, which belongs to the field of biosynthesis. Background technique [0002] Cytosine and guanine are the main base components of nucleic acid. The two form complementary base pairs in the form of hydrogen bonds. This hydrogen bond connection is one of the main forces for maintaining the stability of the DNA double helix structure. In addition to its own physiological functions, it also has a variety of medicinal values. Cytosine is an important intermediate in medicine, mainly used for the synthesis of anti-AIDS and anti-tumor drugs; guanine is also an important compound for the synthesis of compounds with antiviral activity Intermediate, with great industrial production value. [0003] At present, the preparation of cytosine and guanine is generally a chemical synthesis method, using a variety of raw materials and a large amount of solvents in the process, and the reaction condition...
Claims
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