Preparation method of carbohydrate derivative by utilization of glycosyl transferase

A technology of glycosyltransferase and a manufacturing method, which is applied in the field of manufacturing carbohydrate derivatives, can solve the problems of high price of amylase, and achieve the effects of improved functional properties and huge application potential.

Inactive Publication Date: 2017-05-31
BIOLAND BIOCHEM HAIMEN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the price of amylase for the synthesis of α-1~4 structure substances is too expensive

Method used

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  • Preparation method of carbohydrate derivative by utilization of glycosyl transferase
  • Preparation method of carbohydrate derivative by utilization of glycosyl transferase
  • Preparation method of carbohydrate derivative by utilization of glycosyl transferase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The method for producing carbohydrate compound derivatives using glycosyltransferase of the present invention comprises the following steps: adding glycosyltransferase to a solution with a sugar concentration of 0.1M so that the final active concentration of the reaction vessel reaches 0.1U / mL Finally, add at least one glycosyl acceptor selected from the group consisting of 2-amino acid, 3-amino acid, 4-amino acid, hydroquinone and arbutin in the reaction kettle at 15°C to make the final activity The concentration reaches 0.01-3% by mass and reacts. The reaction is formed after 1 hour at 28 ° C. The glucose or fructosyl of the sugar is transferred to the above-mentioned acceptor, and finally acidified by acetic acid to obtain sugar compounds. derivative.

Embodiment 2

[0047] A method for producing carbohydrate compound derivatives utilizing glycosyltransferase, comprising the following steps: adding glycosyltransferase to a solution with a sugar concentration of 0.3M so that the final active concentration of the reactor reaches 0.5 U / mL, and then Add at least one glycosyl acceptor selected from the group consisting of 2-amino acid, 3-amino acid, 4-amino acid, hydroquinone and arbutin in a reaction kettle at 20°C, so that the final active concentration reaches The mass percentage content is 0.01-3% and reacted. The reaction is formed after 8 hours at 32 ° C. The glucose or fructosyl of the sugar is transferred to the above-mentioned acceptor, and finally the sugar compound derivative can be obtained by acetic acidification.

Embodiment 3

[0049] The method for producing carbohydrate compound derivatives using glycosyltransferase of the present invention comprises the following steps: adding glycosyltransferase to a solution with a sugar concentration of 0.5M so that the final active concentration of the reaction vessel reaches 2.0 U / mL Finally, add at least one glycosyl acceptor selected from the group consisting of 2-amino acid, 3-amino acid, 4-amino acid, hydroquinone and arbutin in the reaction kettle at 25°C, so that the final The active concentration reaches 0.01-3% by mass and reacts. The reaction is formed after 10 hours at 34°C. The glucose or fructosyl of the sugar is transferred to the above-mentioned acceptor, and finally the sugar can be obtained by acidification with acetic acid Compound derivatives.

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Abstract

The invention discloses a preparation method of a carbohydrate derivative by the utilization of glycosyl transferase. The preparation method comprises the following steps: adding glycosyl transferase into a solution with sugar concentration of 0.1-1 M to make the final activity concentration of a reaction vessel reach 0.1-3.0 U / mL, adding at least one glycosyl receptor selected from a group consisting of 2-amino acid, 3-amino acid, 4-amino acid, hydroquinone and arbutin into the reaction vessel of 15-50 DEG C to make the final activity concentration reach 0.01-3 wt% and carrying out a reaction at 28-37 DEG C for 1-18 h, letting glucose or fructosyl of sugar be transferred into the above receptor and finally acidifying with acetic acid so as to obtain the carbohydrate derivative. The invention discloses the derivative prepared by the above method. The derivative raises water-solubility, has efficacy of preventing decomposition or oxidation when contacting with light and heat, can be selectively transferred on glucose channels of cytomembrane of blood vessels and brain, and can be effectively used in relevant industries such as food, cosmetic, pharmaceuticals, etc.

Description

technical field [0001] The present invention relates to a method for producing carbohydrate derivatives using glycosyltransferase. [0002] The invention also relates to derivatives obtained by this process. Background technique [0003] Aminophenols are generally important intermediates in the manufacture of sugar compound derivatives, and are also used in the manufacture of dyes and other pharmaceutical intermediates. In addition, 4-aminophenol is easily oxidized into cytotoxic substances in the metabolic process, which can cause various diseases of the blood vessels adjacent to the kidney. (Lock etc., Studies on the mechanism of 4-aminophenol-induced toxicity to renal proximal tubes. Hum. Exp. Toxicol. 12(5), 383-388, 1993.) [0004] The organic synthesis method of 4-aminophenol derivatives has been studied to some extent in the prior art. For example, 4-[2-(5-methyl)]-aminothiazole-2,3-D-tert-butylphenol (Japanese Patent No. 67023 / 1987) and 3-methyl-4-hydroxy- 5-n-pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/44C12P19/18
Inventor 金基洙金荣熙卢勇金庚泰朴智浩陈秀玉
Owner BIOLAND BIOCHEM HAIMEN CO LTD
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