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Alcaligenes faecalis y1 and its application in the control of crop soil-borne diseases

A technology of Alcaligenes faecalis and crops, applied to Alcaligenes faecalis Y1 and its application in the prevention and control of soil-borne diseases of crops, which can solve the problems of unsatisfactory control effects, achieve continuous increase in production and protect the ecological environment of farmland, The effect of promoting production is obvious

Active Publication Date: 2020-03-27
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The control effect is not very satisfactory, and the control effect is only about 80%. Therefore, it is very important to develop bio-fertilizers to prevent and control soil-borne diseases and continuous cropping obstacles

Method used

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  • Alcaligenes faecalis y1 and its application in the control of crop soil-borne diseases
  • Alcaligenes faecalis y1 and its application in the control of crop soil-borne diseases
  • Alcaligenes faecalis y1 and its application in the control of crop soil-borne diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of embodiment 1 Alcaligenes faecalis Y1 bacterial agent

[0033] 1. Isolation and purification of Alcaligenes faecalis Y1

[0034] A strain Y1 that can effectively control sunflower sclerotinia and rust was isolated and purified from farmland soil in Shuangcheng City, Heilongjiang Province, my country.

[0035] According to the bacteriological characteristics of the strain Y1 and the 16S rDNA sequencing results (SEQ ID NO: 1), the strain Y1 was determined to be Alcaligenes faecalis.

[0036] 2. Cultivation of Alcaligenes faecalis Y1

[0037] (1) Composition of medium (kg / t fermentation liquid)

[0038] Soluble starch 10kg, soybean cake powder 10kg, corn flour 5kg, glucose 1kg, sucrose 10kg, yeast powder 5kg, (NH 4 )2 SO 4 3kg, K 2 HPO 4 0.2kg, NaCl 2.5kg, MgSO 4 ·7H 2 O 0.1kg, CaCO 3 0.5kg, FeSO 4 0.001kg, boric acid (1%) 1L, pH7.0, prepared with water.

[0039] (2) Fermentation and cultivation process

[0040] Alcaligenes faecalis Y1 was ...

Embodiment 2

[0045] Example 2 Influence of Alcaligenes faecalis Y1 microbial agent on growth-promoting effect of potted sunflower

[0046] 1. Growth-promoting effect of Y1 bacterial agent on potted sunflower in non-saline-alkaline soil

[0047] The inoculation test of Alcaligenes faecalis Y1 inoculum was carried out under greenhouse conditions. Sunflowers were planted under pot conditions and inoculated with two kinds of biological fertilizer inoculum respectively. The test results showed that the inoculation of microbial agents could increase the biomass of sunflower (Table 1). The biomass of Y1 bacterial agent was the highest, which was 46.0% higher than that of the control (no microbial bacterial agent); the biomass of sunflower inoculated with Alcaligenes faecalis CC1d (isolated in this laboratory) was significantly lower than that of Y1, and it was still 30.1% higher than that of the control. The test results show that the Y1 bacterial agent has a significant effect on promoting the ...

Embodiment 3

[0056] Example 3 Effect of Y1 bacterial agent on tomato output in greenhouse

[0057] The effects of four kinds of bio-fertilizers were studied under the conditions of large-scale use of chemical fertilizers and organic fertilizers. The amount of nitrogen, phosphorus and potassium compound fertilizer (15:15:15) is 83kg / mu, and the amount of organic fertilizer (chicken manure) is 7t / mu. Strains J15 (isolated in our laboratory and identified as Stenotrophomonas sp.), JK24 (isolated in our laboratory and identified as Bacillus megaterium), JK8 (isolated in our laboratory and identified as intermediate shortwave The three strains of Brevundimonas intermedia) were all isolated from the farmland soil of Gongzhuling, Jilin Province by our laboratory, and the field test was carried out together with the strain Y1. Yield results showed that the inoculation of 4 kinds of bio-fertilizers in the greenhouse significantly increased the yield of tomatoes, and the yield of tomatoes with bio-...

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Abstract

The invention provides a strain of Alcaligenes faecalis Y1 and its application in preventing and controlling soil-borne diseases of crops. The preservation number of the said Alcaligenes faecalis Y1 is CGMCC NO.13510, and it is made into a microbial agent that has obvious effects on promoting the growth and production of sunflowers in the field, and its effect is not inhibited by field soil conditions and the use of chemical fertilizers, and can be used in light In high salinity farmland, it is applied together with chemical fertilizers. The fungal agent has a control effect of more than 95% on the rotten disc disease caused by sunflower sclerotin, and a 100% control effect on rust. The microbial agent is of great significance to the prevention and control of soil-borne diseases and continuous cropping obstacles in my country's farmland, the continuous increase of grain production and the protection of the ecological environment of farmland.

Description

technical field [0001] The invention relates to the technical fields of microbiology and plant disease biocontrol, in particular to a strain of Alcaligenes faecalis Y1 and its application in preventing and controlling crop soil-borne diseases. Background technique [0002] With the improvement of crop yield and the development of facility agriculture, the problems of soil-borne diseases and continuous cropping obstacles have become increasingly prominent, and soil pathogenic microorganisms have continued to increase, causing crop yield reduction or even failure. Soil-borne diseases and continuous cropping obstacles have become bottlenecks in the development of safe agricultural production. Continuous cropping for 3 years will reduce yield by 10% to 20%, and crop yield will decrease by 40% to 50% in continuous cropping for 4 to 5 years. Soil-borne diseases of protected vegetables, potatoes, garlic, sunflower, rapeseed, cotton, soybean, rice, wheat, and sugar beet occur frequ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00A01P21/00C05G3/00C05G3/60C05F11/08C12R1/05
CPCA01N63/00C05B7/00C05D9/00C05F11/08C12N1/205C12R2001/05C05F5/002C05F11/00C05C9/00C05D1/00
Inventor 范丙全
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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