TK, ge and gT gene deletion strain of porcine pseudorabies virus variant and application of TK, ge and gT gene deletion strain

Abstract

The invention discloses a TK, ge and gT gene deletion strain of a porcine pseudorabies virus (PRV) variant ZJ01 and application of the TK / ge / gT gene deletion strain. The strain is the TK / ge / gT gene deletion strain ZJ01delta TK / gE / gI (ZJ01R in short) of the PRV ZJ01 strain. According to the in-vitro passage test results, gE / gI gene deletion strains ZJ01delta gE / gI of a recombinant virus ZJ01R and the PRV ZJ01 strain are consistent with cytopathic effect generated by a parental virus ZJ01, and virus titer is similar with the ZJ01 strain; a target gene deletion region of the recombinant virus ZJ01R continuously and stably exists without resilience mutation; according to the pig immunity and attacking protection test, after the recombinant virus ZJ01R is immune, a pig is free of clinical symptoms and pathological changes; after being immunized by 21 days, the pig can generate a PRV neutralizing antibody with relatively high level, and can resist challenging infection of a fatal PRV variant strain ZJ01.

Description

[0001] Technical field: [0002] The invention relates to a porcine pseudorabies virus variant strain used for preparing a vaccine, in particular to a porcine pseudorabies virus variant strain TK, gE and gI gene deletion strain. [0003] Background technique: [0004] Porcine pseudorabies is an important infectious disease of pigs caused by pseudorabies virus (PRV). The main clinical symptoms include: central nervous system disorders in newborn piglets, respiratory symptoms in weaned piglets and finishing pigs, and reproductive disorders in pregnant sows. , has caused huge economic losses to the world's pig farming industry [1-3] . At present, the prevention and control of the disease mainly relies on vaccine immunization. The United States and some European countries have announced that the epidemic of the disease in domestic pigs has been eradicated through gene deletion vaccine immunization and purification technology [4] . In the past 30 years, my country has widely used...

AI Technical Summary

Problems solved by technology

However, since 2011, many pig farms immunized with live PRV vaccines in my country have had outbreaks of the disease again, mainly manifested as neurological symptoms and death of piglets, respiratory symptoms of growing pigs, abortion of pregnant sows, stillbirth, weak piglets, etc. present new challenges

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