Efficient identification method for drought resistance ability of alfalfa varieties

Abstract

The invention discloses an efficient identification method for the drought resistance ability of alfalfa varieties. The method comprises the following steps: carrying out polyethylene glycol PEG-6,000-simulated drought stress treatment; measuring physiological indexes with the most sensitive change and sensitive thresholds of the alfalfa varieties with different drought resistance levels when responding to different stress intensity; building sensitive physiological index groups and sensitive threshold index systems corresponding to the drought resistance levels of the alfalfa varieties; and dividing to-be-identified alfalfa varieties into high drought resistance, medium drought resistance and low drought resistance. According to the identification method, when the drought stress is simulated by different concentration of PEG-6,000, the sensitive physiological index groups and the sensitive threshold index systems corresponding to the drought resistance levels are built on the basis of physiological indexes which are closely related to the alfalfa varieties with different drought resistance levels and the sensitive thresholds as identification bases, and the drought resistance ability of the alfalfa is graded, so that the comprehensive evaluation result of the drought resistance of the alfalfa is improved, the method has good operability and reliability, identification of the drought resistance can be completed in a laboratory, the conditions are easy to control and the cost is low.

Description

technical field [0001] The invention relates to the technical field of plant drought resistance identification, in particular to an efficient identification method for the drought resistance ability of alfalfa varieties. Background technique [0002] The drought resistance identification of alfalfa is often accompanied by the introduction and breeding of alfalfa. Usually, alfalfa varieties with different drought resistance are cultivated in the field or indoors, and the seedlings are grown or treated for a certain period of time under normal water conditions (control) or drought stress with increased stress intensity. The changes of its morphology and physiological indexes after the impact, through the correlation analysis of the two, make a comprehensive evaluation of its drought resistance. In the identification of drought resistance of alfalfa, the focus is on the selection of drought resistance indicators, which can be summarized into four categories: morphological indi...

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Problems solved by technology

[0004] When using greenhouse pot plant simulated drought environment or PEG simulated drought combined with determination of drought resistance physiological indicators to evaluate the drought resistance ability of alfalfa seedlings, the evaluation method is the comprehensive evaluation under the unified index system, only considering the effect of the same drought resistance physiological index category of alfalfa with different drought resistance levels on drought There are differences in the sensitivity to stress, but the differences in the sensitivity physiological indicators of alfalfa with different drought resistance levels under different stress intensities are not considered. Therefore, the selected drought resistance physiological indicators lack specificity and representativeness, which in turn affects the drought resistance of alfalfa. The accuracy of comprehensive evaluation results