Extraction method for medlar peptide

An extraction method and wolfberry technology, which are applied in the preparation methods of peptides, chemical instruments and methods, peptides and other directions, can solve the problems of difficulty in achieving the extraction effect of wolfberry polypeptides, and achieve the effects of easy large-scale promotion, low cost, and improved utilization.

Inactive Publication Date: 2017-06-20
GUOTAI BIOLOGICAL TECH BEIJING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because the existing extraction methods are not optimized in terms of solvent usage, decoction time, and decoction times, it is often difficult to achieve the best extraction effect of Lycium barbarum polypeptide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The extraction method of the wolfberry peptide of the present embodiment is prepared according to the following steps:

[0031] Step 1, after soaking 100Kg wolfberry with a calcium hypochlorite solution with a concentration of 0.06wt%, soaking with a sodium chloride solution with a concentration of 0.6% for 20 minutes, and rinsing with clear water twice to obtain preliminary sterilized wolfberry;

[0032] Step 2: Sterilize the preliminarily sterilized wolfberry at 100°C for 8 seconds, cool to room temperature to obtain sterilized wolfberry, then transfer the sterilized wolfberry to a beater, add distilled water with a mass volume ratio of 1:3 to the sterilized wolfberry, and beat 15min, pressure filtration (aperture is 110 orders), obtain wolfberry juice and wolfberry slag respectively;

[0033] Step 3, adding casein with a mass ratio of 1:0.05 to the wolfberry slag to the wolfberry slag, adding phospholipids with a mass ratio of 1:0.034 to the wolfberry slag, and vibra...

Embodiment 2

[0040] The extraction method of the wolfberry peptide of the present embodiment is prepared according to the following steps:

[0041] Step 1, after soaking 15Kg wolfberry with a calcium hypochlorite solution with a concentration of 0.06wt%, soaked with a sodium chloride solution with a concentration of 0.5% for 30 minutes, and rinsed twice with clear water to obtain preliminary sterilized wolfberry;

[0042] Step 2: Sterilize the preliminarily sterilized wolfberry at 100°C for 6 seconds, cool to room temperature to obtain sterilized wolfberry, then transfer the sterilized wolfberry to a beater, add distilled water with a mass volume ratio of 1:2.8 to the sterilized wolfberry, and beat 18min, filter press (aperture is 120 orders), obtain wolfberry juice and wolfberry slag respectively;

[0043] Step 3, adding casein with a mass ratio of 1:0.04 to the wolfberry slag to the wolfberry slag, adding phospholipids with a mass ratio of 1:0.03 to the wolfberry slag, and vibrating at r...

Embodiment 3

[0050] The extraction method of the wolfberry peptide of the present embodiment is prepared according to the following steps:

[0051] Step 1, after soaking 800Kg wolfberry with a calcium hypochlorite solution with a concentration of 0.06wt%, soak it with a sodium chloride solution with a concentration of 0.8% for 26 minutes, and rinse it with clear water for 3 times to obtain preliminary sterilized wolfberry;

[0052] Step 2: Sterilize the preliminarily sterilized wolfberry at 100°C for 5 seconds, cool to room temperature to obtain sterilized wolfberry, then transfer the sterilized wolfberry to a beater, add distilled water with a mass volume ratio of 1:2.6 to the sterilized wolfberry, and beat 24min, pressure filtration (aperture is 100 orders), obtain wolfberry juice and wolfberry slag respectively;

[0053] Step 3, adding casein with a mass ratio of 1:0.045 to the wolfberry slag to the wolfberry slag, adding phospholipids with a mass ratio of 1:0.04 to the wolfberry slag, an...

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Abstract

The invention discloses an extraction method for medlar peptide. By choosing fresh high-quality medlar and applying biological enzymolysis technology, through a process flow of preprocessing, juice preparation, low-temperature immersion, hydrolyzation, filtration, concentration and spray drying, the extraction method can successfully convert active ingredients in the medlar into a small-molecule peptide type substance and efficiently extract out the small-molecule peptide type substance. By improving the preparation process and carrying out enzymolysis and filtration on the material multiple times, the extraction method for medlar peptide disclosed by the invention not only increases the utilization rate of medlar, but also increases the extraction rate of medlar peptide, and has the characteristics of low cost, high yield and easiness in large-scale popularization.

Description

technical field [0001] The invention relates to the technical field of polypeptide extraction, in particular to a method for extracting wolfberry peptide. Background technique [0002] Lycium barbarum, also known as wolfberry fruit and red earrings, is the mature fruit of Lycium barbarum, a small shrub in the Solanaceae family. It was first recorded in "Shen Nong's Materia Medica", saying that it is "long-term clothing, light body, not old, cold and heat-resistant", and has the effect of delaying aging and anti-aging , also known as "Que Laozi". Modern pharmacology shows that wolfberry has the functions of improving the body's immune function, anti-oxidation, anti-aging, protecting the liver, increasing white blood cells, etc., and is listed as an important nourishing drug. In addition, the Lycium barbarum polypeptide in Lycium barbarum is one of its main active ingredients and has a great market prospect. [0003] At present, Pueraria lobata products on the market are mos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/34
CPCC07K1/145C07K1/34C12P21/06
Inventor 张恒
Owner GUOTAI BIOLOGICAL TECH BEIJING CO LTD
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